Fluorescent Protein
RSS feedHandbook of Optical Filters for Fluorescence Microscopy
Fluorescence microscopy and other light-based applications require optical filters that have demanding spectral and physical characteristics. Often, these characteristics are application-specific and…Read articleFluorescent Proteins – Introduction and Photo Spectral Characteristics
The prospects of fluorescence microscopy changed dramatically with the discovery of fluorescent proteins in the 1950s. The starting point was the detection of the jellyfish Aequorea victoria green…Read articleModern Fluorescent Proteins and their Biological Applications
Here we present two review articles on fluorescent proteins and their biological applications. These first article reviews our current knowledge of blue, green, and red chromophore formation in…Read articleCell Cultures and Laser Microdissection
Many of the discoveries that are now being made in cell division and differentiation, the relationships between single cells and cell organelles, treatments of cells with pharmaceutic substances, etc.…Read articleA Genetically Encoded Tag for Correlated Light and Electron Microscopy of Intact Cells, Tissues, and Organisms
Electron microscopy (EM) achieves the highest spatial resolution in protein localization, but specific protein EM labeling has lacked generally applicable genetically encoded tags for in situ…Read articleAn Introduction to Fluorescence
Fluorescence is widely used in microscopy and an important tool for observing the distribution of specific molecules. Most molecules in cells do not fluoresce. They therefore have to be marked with…Read articleA mTurquoise-Based cAMP Sensor for Both FLIM and Ratiometric Read-Out Has Improved Dynamic Range
FRET-based sensors for cyclic Adenosine Mono Phosphate (cAMP) have revolutionized the way in which this important intracellular messenger is studied. The currently prevailing sensors consist of the…Read articleChoose Your Excitation Wavelength
Although time correlated single photon counting (TCSPC) is the method of choice for fluorescence lifetime quantification, it requires dedicated instrumentation including a pulsed laser source, a…Read articleATP Changes the Fluorescence Lifetime of Cyan Fluorescent Protein via an Interaction with His148
This study employed fluorescence lifetime spectroscopy to investigate the influence of ATP (adenosine triphosphate) on the fluorescence intensities of yellow and cyan fluorescent proteins (YFP/CFP)…Read articleWhat is an OPO?
Multiphoton microscopy with