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A New Filtering Technique for Removing Anti-Stokes Emission Background in Gated CW-STED Microscopy
Stimulated emission depletion (STED) microscopy is a prominent approach of super-resolution optical microscopy, which allows cellular imaging with so far unprecedented unlimited spatial resolution.…Read article
Live-Cell Imaging Evolves to Find New Niches
Since its introduction in the 1600s, improvements in microscope technology have continually broadened the types of cells and cellular processes that can be studied. Advances in automation have made…Read article
Super-resolution Microscopy and the Third Dimension
Optical imaging devices have a finite depth of field and diffraction limited resolution. The depth of field problem was tackled first with confocal microscopes, diffraction unlimited resolution is…Read article
Interview with Stefan Hell: Breaking Nanoscale Boundaries
Stefan Hell is credited with having conceived and applied the first viable concept for breaking Abbe’s diffraction limit in a light-focusing microscope. He is a scientific member of the Max Planck…Read article
STED Microscopy: A New Chapter in Light Imaging
When Stefan Hell invented a technique that pushed beyond the diffraction limit of light for the first time, a new field of nanoscale imaging was born. Now laboratories around the world are using …Read article
Towards Real-time Image Deconvolution: Application to Confocal and STED Microscopy
Although deconvolution can improve the quality of any type of microscope, the high computational time required has so far limited its massive spreading. Here we demonstrate the ability of the…Read articleSingle-Wavelength Two-Photon Excitation-stimulated Emission Depletion (SW2PE-STED) Superresolution Imaging
Two-photon microscopy, multiphoton microcopy and super-resolution imaging. We developed a new class of two-photon excitation–stimulated emission depletion (2PE-STED) optical microscope. In this…Read article
Watching Molecule Movements in Live Cells
The newly developed STED-RICS microscopy method records rapid movements of molecules in live samples. By combining raster image correlation spectroscopy (RICS) with STED fluorescence microscopy,…Read article
New Labeling Tools Can Help to Realize the Full Potential of Super-Resolution Microscopy
Since super-resolution microscopy techniques revolutionized the concept of light microscopy by overcoming the physical diffraction limit, STED microscopy and other super-resolution techniques have…Read article
Abstracts of the 3rd European Super-Resolution User-Club Meeting
The 3rd meeting of the Leica Super-Resolution User Club was held from June 17th to 19th, 2013 in collaboration with Alberto Diaspro and the Italian Institute of Technology (IIT) in Genoa. Confocal and…Read articleSTED Nanoscopy with Time-Gated Detection: Theoretical and Experimental Aspects
In a stimulated emission depletion (STED) microscope the region in which fluorescence markers can emit spontaneously shrinks with continued STED beam action after a singular excitation event. This…Read article
STED and GSDIM: Diffraction Unlimited Resolution for all Types of Fluorescence Imaging
This article gives an overview of two different types of superresolution techniques. Stimulated emission depletion (STED) microscopy is a versatile and fast method that is based on point scanning…Read article
Gates Open for Improved Confocal Fluorescence and Super-Resolution STED
True confocal microscope systems feature single-point illumination and single-point detection. The method is called "optical sectioning" since the generated image contains only information from the…Read article
Mitochondrial DNA Molecules are Packaged Individually
At the Max Planck Institute for Biology of Ageing in Cologne founded in 2008, three departments are working on the molecular, physiological and evolutionary mechanisms of the ageing of cells, tissue…Read article
Abstracts of the 2nd European Super-Resolution User-Club Meeting
The 2nd meeting of the Leica Super-resolution User club was held from September 25 to 27, 2012 in collaboration with the Science for Life Laboratory at the Karolinska Institute, Stockholm, Sweden. …Read article
