Cell Biology
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Nobel Prize 2012 in Chemistry for Studies of G-Protein-Coupled Receptors
The Royal Swedish Academy of Sciences has decided to award the Nobel Prize in Chemistry for 2012 to Robert J. Lefkowitz and Brian K. Kobilka for studies of G-protein–coupled receptors.Read article
Taking the Long View
In exploring how embryos take shape, John Wallingford has identified a key pathway involved in vertebrate development – and human disease.Read article
Order versus Disorder
In modern biomaterial design the generation of an environment mimicking some of the extracellular matrix features is envisaged to support molecular cross-talk between cells and scaffolds during tissue…Read article
TIRF Microscopy Explores Cellular Transport Processes
Due to their special role in organ function and the exchange of biological components some body cells developed certain polarization characteristics. These are reflected in differences of their plasma…Read article
How to Study Protein Recruitment to DNA Lesions by a Combination of UV Laser and White Light Laser
Understanding how DNA lesions are optimally repaired is of functional significance, especially from the view of genome karyotype stability.Read article
The New Repository on the Block
The need for data validation and accessibility has never been greater than it is today. We are inundated with information from a multitude of resources, but how can we easily evaluate the accuracy of…Read article
Quantitative Imaging in Cell Biology: Light Sheet Microscopy
This chapter introduces the concept of light sheet microscopy along with practical advice on how to design and build such an instrument. Selective plane illumination microscopy is presented as an…Read article
Organotypic Cerebellar Cultures: Apoptotic Challenges and Detection
Organotypic cultures of neuronal tissue were first introduced by Hogue in 1947 and have constituted a major breakthrough in the field of neuroscience. Since then, the technique was developed further…Read article
TIRF Microscopy of the Apical Membrane of Polarized Epithelial Cells
Application of TIRF microscopy (Total Internal Reflection Fluorescence) allows the visualization of structures at the apical surface of polarized epithelial cells that have been hidden in conventional…Read article
High Data Output and Automated 3D Correlative Light–Electron Microscopy Method
Correlative light/electron microscopy (CLEM) allows the simultaneous observation of a given subcellular structure by fluorescence light microscopy (FLM) and electron microscopy. The use of this…Read article
Exploring Cell Logistics
Using TIRF microscopy, scientists have been able to take a closer look at intracellular transport processes with the example of the galactose-binding protein Galectin-3, which has been identified as a…Read article