Sample Preparation
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Symmetric Synapse - Clathrin Coated Endocytosis Pit in the Postsynaptic Dendrite
Application Note for Leica EM ICE - WT hippocampal neurons were plated at a density of 80,000 cells/cm2 on 6 mm sapphire disks for 14 days. Sample were frozen using a high-pressure freezer (Leica EM…Read article
Electron Microscopy of High Pressure Frozen and Freeze Substituted Arabidopsis Thaliana Root Tips Cells
Application Note for Leica EM AFS2 - Arabidopsis thaliana roots (mutant PIN1pro:PIN1-GFP;bex5-1) were excised, immersed in 20% (w/v) BSA and frozen immediately in a high-pressure freezer. Freeze…Read article
Cross Sectioning of Oil Shale Rock
Application Note for Leica TIC 3X - High quality sample preparation of large area to investigate the sample in the SEM. For the mechanical preparation step diamond lapping foils of 9μm subsequently…Read articleCryo-SEM Imaging of Latex Paint
Application Note for Leica EM VCT100, Leica EM ACE600 - A thin layer of latex paint was spread on a clean, scored, silicon chip. The sample was immediately plunge frozen in liquid ethane and…Read article
Cross Sectioning of Rubber (Tire)
Application Note for Leica EM TIC 3X - Ion beam slope cutting is a method that can achieve cross sections of soft materials or material combinations consisting of hard and soft components.Read article
Water Flea Protocol
Application Note for Leica EM CPD300 - Critical point drying of water flea with subsequent gold coating and SEM-Analysis to detect finde surface structures.Read article
Workflows & Protocols: Plant Laser Microdissection
During Leica workshops for LMD users in Brazil, hosted by the Federal University of Paraná/UFPR (UFPR) at the Centro de Energia Nuclear na Agricultura/USP (CENA), the power of laser microdissection…Read article
Human Blood Cells Protocol
Application Note for Leica EM CPD300 - Life Science Research. Species: Human (Homo sapiens) Critical point drying of human blood with subsequent platinum / palladium coating and SEM analysis.Read article
Cross Sectioning of Basalt Fibres
Application Note for Leica EM TIC 3X - Material Research. Purpose: The fibres are embedded in a soft matrix. That makes it difficult to prepare a cross section. Goal: Cross section of the basalt…Read article
Workflows & Protocols: Laser Microdissection for Pathology and Cancer Research
Tumor development results from mutations in our DNA. For their deeper analysis, cancer researchers have to dissect the relevant tissue areas. Here we report the reason why laser microdissection is a…Read article
Improvement of Metallic Thin Films for HR-SEM by Using DC Magnetron Sputter Coater
Preparation techniques, like several kinds of coating methods play an important role for high resolution scanning electron microscopy (HR-SEM). Nonconductive sample like biological and synthetic…Read articleHow to do a Proper Cell Culture Quick Check
In order to successfully work with mammalian cell lines, they must be grown under controlled conditions and require their own specific growth medium. In addition, to guarantee consistency their growth…Read article
Triple-beam Ar-Ion-Milling with a Rotary Stage to Decorate Grain Boundaries and Substructures in Rock Salt
Decoration of grain boundaries in polycrystalline rocks has a long tradition in Structural Geology as in a monomineralic rock the recrystallized grain size is a good indicator for the paleostress…Read article
TEM Sample Preparation Made Easy - Prepare TEM Specimen by Broad Beam Argon Ion Milling
Quantitative and analytical analysis at high spatial resolution places stringent demands on the quality of the produced TEM specimens. Pristine and high-quality samples are indispensible for atomic…Read article
Workflows & Protocols: Connecting Microscopy and Molecular Biology in Neuroscience
The main topic during this course was how to apply laser microdissection in neuroscience. Leica specialists demonstrated why laser microdissection is a suitable techniques for brain investigation as…Read article