STED
RSS feed
Visualization of the Natural Killer Cell Immune Synapse by Super-Resolution Nanoscopy
Natural killer (NK) cells are innate immune effectors that recognize and kill virally infected and tumorigenic cells. Central to their effector function is the formation of an immunological synapse…Read article
Sharp Live Images from the Mouse Brain
To explore the most intricate structures of the brain in order to decipher how it functions – Stefan Hell’s team of researchers at the Max Planck Institute for Biophysical Chemistry in Göttingen has…Read article
Dual Channel STED Nanoscopy of Lytic Granules on Actin Filaments in Natural Killer Cells
Natural killer (NK) cells are innate immune effectors that eliminate diseased and tumorigenic targets through the directed secretion of specialized secretory lysosomes, termed lytic granules. This…Read article
Live-cell Imaging Techniques
The understanding of complex and/or fast cellular dynamics is an important step for exploring biological processes. Therefore, today’s life science research is increasingly focusing on dynamic…Read article
Videos on STED Microscopy and its Inventor Stefan Hell
Here we present an collection of videos telling the story of the invention of nanometer-scale microscopy by Stefan Hell and explaining the basic principle of STED microscopy.Read article
Subcellular Localization of AKT and Tubulin using Super-Resolution Microscopy
Stimulated Emission Depletion microscopy, or STED nanoscopy, is a technique that uses the non-linear de-excitation of fluorescent dyes to overcome the resolution limit imposed by diffraction…Read article
Two-color STED Microscopy of Living Synapses using a Single Laser-beam Pair
The advent of superresolution microscopy has opened up new research opportunities into dynamic processes at the nanoscale inside living biological specimens. This is particularly true for synapses,…Read article
Modern Fluorescent Proteins and their Biological Applications
Here we present two review articles on fluorescent proteins and their biological applications. These first article reviews our current knowledge of blue, green, and red chromophore formation in…Read article
Applications, Labeling Strategies and Fluorophores for Super-Resolution
The free online webinar on super-resolution presented by Leica Microsystems in association with Microscopy & Analysis took place on Tuesday, 15 November 2011. Register and view the webinar on demand.Read article
Neurobiology and Microscopy
Neurobiology, the science of nerves and the brain, has mainly been driven forward in the last 200 years by microscopic investigations. The structures of cellular and subcellular structures,…Read article
Natural Killer Cell Lytic Granule Secretion Occurs through a Pervasive Actin Network at the Immune Synapse
Accumulation of filamentous actin (F-actin) at the immunological synapse (IS) is a prerequisite for the cytotoxic function of natural killer (NK) cells. Subsequent to reorganization of the actin…Read article
STED Nanoscopy of Actin Dynamics in Synapses deep inside Living Brain Slices
It is difficult to investigate the mechanisms that mediate long-term changes in synapse function because synapses are small and deeply embedded inside brain tissue. Although recent fluorescence…Read article
Nanoscopy in a Living Multicellular Organism Expressing GFP
We report superresolution fluorescence microscopy in an intact living organism, namely Caenorhabditis elegans nematodes expressing green fluorescent protein (GFP)-fusion proteins. We also…Read article
Restless Receptors
Synapses are the switch-points in our brain for information transmission, learning and memory. News studies and developments of imaging techniques have provided new insights into the dynamics of…Read article
The Missing Link to the Nanocosm of Life
Fully understanding the functionality and complexity of the human central nervous system remains one of the major open questions in modern science. Stimulated emission depletion microscopy (STED) can…Read article