Sample Preparation
RSS feedWorkflows & Protocols: How to Isolate Individual Chromosomes with Laser Microdissection
During the first Leica Workshop in Brazil, at the Centro de Energia Nuclear na Agricultura/USP (CENA), the participants learned how to prepare samples for laser microdissection (LMD) using a cryotome.…Read articleElectron Microscopy Sample Preparation: “The Future is Cold, Dynamic and Hybrid”
In 2014, the renowned Electron Microscopy for Materials Science (EMAT) research lab at the University Antwerp, Belgium, and Leica Microsystems started a fruitful collaboration to establish a Leica…Read articleA Good Place for Materials Scientists and Mineralogists to prepare their EM Samples
In June 2014, the Institut de Minéralogie, de Physique des Matériaux et de Cosmochimie (IMPMC) of the Université Pierre et Marie Curie in Paris became a Leica reference lab for EM Sample Preparation…Read articleWorkflows & Protocols: How to Use a Leica Laser Microdissection System and Qiagen Kits for Successful RNA Analysis
Laser Microdissection (LMD) allows isolating individual cells or chromosomes and is a well established technique for sample preparation prior downstream analysis of the nucleic acid content via PCR or…Read articleCritical-point Drying for the Preparation of Biological Samples for MicroCT Analysis
X-ray micro-computed tomography (micro-CT) is a routinely applied non-invasive technique for the investigation of the internal anatomy and morphology of organisms. As a result of a micro-CT scan a…Read articleTransmission Electron Microscopy Sample Preparation Protocols for the Ultrastructural Study of Cysts of Free-living Protozoa
Cysts of free-living protozoa have an impact on the ecology and epidemiology of bacteria because they may act as a transmission vector or shelter the bacteria against hash environmental conditions.…Read articleIntravenous Microinjections of Zebrafish Larvae to Study Acute Kidney Injury
We describe a technique of microinjecting the aminoglycoside, gentamicin, into 2 days post-fetilization (dpf) zebrafish larvae to induce acute kidney injury (AKI). We also describe a method for whole…Read articleHigh Quality Sample Preparation for EBSD Analysis by Broad Ion Beam Milling
Electron Backscatter Diffraction technique (EBSD) is known as a "surface" technique because electron diffraction is generated within a few tens of nanometers of the sample surface. Therefore, the…Read articleCorrelative In-Resin Super-Resolution and Electron Microscopy Using Standard Fluorescent Proteins
We introduce a method for correlative in-resin super-resolution fluorescence and electron microscopy (EM) of biological structures in mammalian culture cells. Cryo-fixed resin embedded samples offer…Read articlePreparation of Small Disk-shaped Samples for Capacitance and Pyroelectric Current Measurements
Capacitance and pyroelectric current measurements are best suited to determine important dielectric properties of materials, e.g. the relative dielectric constant, εr, and the remnant electric…Read articleCorrelative Light and Electron Microscopy – Get Your Free CLEM e-Book for Download
The urge to go deeper into the microscopic world has led researchers to combine the versatility of the light microscopy (