Multiphoton Imaging
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How to Quantify Label-free Blood Flow
The application note “Label-free Blood Flow Quantification” is a guide for data acquisition and analysis of blood flow using label-free third harmonic generation (THG) imaging.Read article
Multiphoton Microscopy Publication List
Multiphoton Microscopy is an advanced technique for imaging thick samples. Applications range from the visualization of the complex architecture of the whole brain to the study of tumor development…Read article
Principles of Multiphoton Microscopy for Deep Tissue Imaging
Basics of multiphoton microscopy. This tutorial explains the principles of multiphoton microscopy for deep tissue imaging. Multiphoton microscopy uses excitation wavelengths in the infrared taking…Read article
Step by Step Guide to the Molecular Basics of CARS Microscopy
CARS (Coherent Anti-Stokes Raman Scattering) microscopy is a dye-free method which images structures by displaying the characteristic intrinsic vibrational contrast of their molecules. The crucial…Read article
Chat with Dr. Thomas Mathivet about In Vivo Cardiovascular Imaging of the Brain
Dr. Thomas Mathivet, working at the Cardiovascular Institute in Paris, France, was interviewed by Dr. Luis Alvarez, Product Application Manager, during Leica Microsystems Meets Science 2019 in…Read article
Coherent Raman Scattering Microscopy Publication List
CRS (Coherent Raman Scattering) microscopy is an umbrella term for label-free methods that image biological structures by exploiting the characteristic, intrinsic vibrational contrast of their…Read article
Image Gallery: Tunable Multicolor Deep In Vivo Imaging
Today’s life science research focusses on complex biological processes, such as the causes of cancer and other human diseases. A deep look into tissues and living specimens is vital to understanding…Read article
Direct visualization of the arterial wall water permeability barrier using CARS microscopy
The artery wall is equipped with a water permeation barrier that allows blood to flow at high pressure without significant water leak. The precise location of this barrier is unknown despite its…Read article
Which Sensor is the Best for Confocal Imaging?
The Hybrid Photodetectors (HyD) are! Why that is the case is explained in this short Science Lab article.Read article
High-Resolution 3D Imaging of Whole Organ after Clearing
Zebrafish testis has become a powerful model for reproductive biology of teleostean fishes and other vertebrates and encompasses multiple applications in applied and basic research. Many studies have…Read article
Multiphoton Microscopy – a Satisfied Wish List
The colorful picture shows colon tumor cells, fluorescently labelled and lineage traced from a multicolor tracer. The gray color codes for the second harmonic generation (SHG) signal from Collagen 1.…Read article
Mission Impossible Accomplished: Tunable Colors for Non-descanning Detection
Leica Microsystems’ 4Tune detector, the key component of the SP8 DIVE Deep In Vivo Explorer, provides spectrally tunable image recording with non-descanning detection. An innovative solution for…Read article
Laser Beam Shaping for Multicolor Multiphoton Microscopy
Multiphoton Microscopy is one of the current hot topics in life science research. The new Leica TCS SP8 DIVE from Leica Microsystems presents a series of beneficial new innovations, including a freely…Read article
Five Questions Asked: Prof. Dr. Jacco van Rheenen speaks about the most important considerations when imaging deep into mouse tissue
When operating a confocal microscope, or when discussing features and parameters of such a device, we inescapably mention the pinhole and its diameter. This short introductory document is meant to…Read article
Human NK Cell Development Requires CD56-mediated Motility and Formation of the Developmental Synapse
While distinct stages of natural killer (NK) cell development have been defined, the molecular interactions that shape human NK cell maturation are poorly understood. Here we define intercellular…Read article