High Pressure Freezing
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Video Tutorials: Filling and Assembling of Different Carriers for High-Pressure Freezing
High pressure freezing (HPF) is a cryo-fixation method primarily for biological samples, but also for a variety of non-biological materials. It is a technique that yields optimal preservation in many…Read article
Brief Introduction to Freeze Fracture and Etching
Freeze fracture describes the technique of breaking a frozen specimen to reveal internal structures. Freeze etching is the sublimation of surface ice under vacuum to reveal details of the fractured…Read article
Brief Introduction to Freeze Substitution
Freeze-substitution is a process of dehydration, performed at temperatures low enough to avoid the formation of ice crystals and to circumvent the damaging effects observed after ambient-temperature…Read article
Brief Introduction to High-Pressure Freezing
Water is the most abundant cellular constituent and therefore important for preserving cellular ultra-structure. Currently the only way to fix cellular constituents without introducing significant…Read article