Calcium Imaging
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Super-Resolution Microscopy of the Synaptic Active Zone
At the presynaptic active zone (AZ) a variety of specialized proteins are assembled to complex architectures, which set the basis for speed, precision and plasticity of synaptic transmission.…Read article
Progressive Glucose Stimulation of Islet Beta Cells Reveals a Transition From Segregated to Integrated Modular Functional Connectivity Patterns
Collective beta cell activity in islets of Langerhans is critical for the supply of insulin within an organism. In order to get a detailed insight into the functional organization of the syncytium, we…Read article
Imaging Pheromone Sensing in a Mouse Vomeronasal Acute Tissue Slice Preparation
In mice, the ability to detect pheromones is principally mediated by the vomeronasal organ (VNO). Here, an acute tissue slice preparation of VNO for performing calcium imaging is described. This…Read article
Glucose-Stimulated Calcium Dynamics in Islets of Langerhans in Acute Mouse Pancreas Tissue Slices
In endocrine cells within islets of Langerhans calcium ions couple cell stimulation to hormone secretion. Since the advent of modern fluorimetry, numerous in vitro studies employing primarily isolated…Read article
Functional Connectivity in Islets of Langerhans from Mouse Pancreas Tissue Slices
We propose a network representation of electrically coupled beta cells in islets of Langerhans. Beta cells are functionally connected on the basis of correlations between calcium dynamics of…Read article
The Relationship Between Membrane Potential and Calcium Dynamics in Glucose-Stimulated Beta Cell Syncytium in Acute Mouse Pancreas Tissue Slices
Oscillatory electrical activity is regarded as a hallmark of the pancreatic beta cell glucose-dependent excitability pattern. Electrophysiologically recorded membrane potential oscillations in beta…Read article