Confocal Microscopy
View on Science LabFind the Needle in the Haystack
The obvious has been explored. These days, biologists strive to identify and analyze hidden and rare events. The task is tackled by automatically screening large numbers of objects – typically growing…Read articlePinhole Controls Optical Slicing
True confocal scanning microscopes (TCS) use a variable detection pinhole. Good optical sectioning tries to use just the inner core of the PSF.Read articleConfocal Optical Section Thickness
Confocal microscopes are employed to optically slice comparably thick samples.Read articleConfocal Microscopy - Optical Path
“Confocal Microscopy” refers to a particular optical microscope that allows recording optical sections. Optical sectioning is achieved in a confocal system by illuminating and observing a single…Read articleChoose Your Excitation Wavelength
Although time correlated single photon counting (TCSPC) is the method of choice for fluorescence lifetime quantification, it requires dedicated instrumentation including a pulsed laser source, a…Read articleCARS and Confocal
The most important drawback of single-photon and multiphoton confocal microscopy is the need to label the specimen. CARS (Coherent Anti-Stokes Raman Spectroscopy) addresses this issue because it is…Read articleExploring the Concert of Neuronal Activities
Brain research using Confocal and Multiphoton Microscopy. Using imaging techniques such as confocal and two-photon microscopy, neuronal dendritic arborization of neurons and their synaptic…Read articleDeep Tissue Imaging
Developmental biology using Multiphoton microscopy with