STED
View on Science Lab
A Versatile Palette of Fluorescent Probes
Researchers at the Max Planck Institute for Medical Research in Heidelberg have developed a general strategy to synthesize live-cell compatible fluorogenic probes, and the result are the new MaP (Max…Read article
STED sheds insight on mitochondrial protein synthesis
In this paper the authors have adapted a click chemistry-based method coupled with stimulated emission depletion nanoscopy (STED) to address these questions reporting that, in human cells in culture,…Read article
Mitochondrial dynamics quantitatively revealed by STED nanoscopy
Mitochondria are vital subcellular organelles, which can generate energy to support the entire lifecycle of biological cells. However, conventional fluorescence microscopy cannot provide a high enough…Read article
Benefits of Combining STED and Lifetime
In this interview, Professor Alberto Diaspro talks about the advantages of the White Light Laser and the TauSTED capabilities of STELLARIS 8 STED. He speaks about his experience with the confocal…Read article
Download TauSTED Infographic
TauSTED combines the optical signals from STED and the physical information from fluorescence lifetime at confocal speeds. Outstanding STED resolution and image quality is achieved due to background…Read article
Visualization of Submitochondrial Protein Distributions
By allowing visualization of submitochondrial protein distributions, fluorescence nanoscopy offers significant advantages for understanding cell death control. How cells manage, and control…Read article
DNA Replication in Cancer Cells
DNA synthesis can be impeded by collisions between the DNA replication machinery and co-transcriptional R-loops leading to a major source of genomic instability in cancer cells. In this paper we…Read article
Benefits of TauContrast to Image Complex Samples
In this interview, Dr. Timo Zimmermann talks about his experience with the application of TauSense tools and their potential for the investigation of demanding samples such as thick samples or…Read article
Time-resolved STED microscopy
Introduced more than 30 years ago, stimulated emission depletion (STED) microscopy has raised to a standard and widely used method for imaging in the life sciences. Thanks to continuous technological…Read article
New Light Shed on the Nanodomain Organization of the Endoplasmic Reticulum (ER)
The endoplasmic reticulum (ER) is a continuous membrane organelle in charge of protein synthesis, lipid synthesis and detoxification. The ER structure is described in terms of smooth peripheral…Read article
The Guide to STED Sample Preparation
This guide is intended to help users optimize sample preparation for stimulated emission depletion (STED) nanoscopy, specifically when using the TCS SP8 STED 3X nanoscope from Leica Microsystems. It…Read article
Phasor Analysis for FLIM (Fluorescence Lifetime Imaging Microscopy)
The Phasor analysis approach to analyze fluorescence lifetime does not require any fitting. Phasor FLIM (fluorescence lifetime imaging microscopy) provides a 2D graphical view of lifetime…Read article
Improve Workflow in Kidney Research with 3D STED Deep Nanoscopy
Visualize the ultrastructure of the glomerular filtration barrier. The combination of optical clearing and nanoscopy allows to study the glomerular filtration barrier at the nanoscale. The Leica TCS…Read article
Extending Nanoscopy Possibilities with STED and exchangeable fluorophores
When it comes to STED Nanoscopy, keeping high signal-to-noise is key to achieve the best possible resolution in fixed and living cells. This can be challenging in the case of experiments in 3D and/or…Read article
Simultaneously Measuring Image Features and Resolution in Live-Cell STED Images
Reliable interpretation and quantification of cellular features in fluorescence microscopy requires an accurate estimate of microscope resolution. This is typically obtained by measuring the image of…Read article
Observing Malaria Infection at the Right Spot in the Human Host
Malaria is a life-threatening disease transmitted through the bites of mosquitoes infected with protozoan parasites. The most common and dangerous type of malaria is caused by the parasite Plasmodium…Read article
Super-resolved STED spectroscopy
Molecular interactions are key in cellular signalling. They are often ruled or rendered by the mobility of the involved molecules.Read article
STED Nanoscopy at the forefront of cancer research
Alison Dun is the postdoctoral facility manager for the Edinburgh Super-Resolution Imaging Consortium (ESRIC), Heriot-Watt University, Edinburgh, UK. She has used a large range of microscope…Read article