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A New Filtering Technique for Removing Anti-Stokes Emission Background in Gated CW-STED Microscopy
Stimulated emission depletion (STED) microscopy is a prominent approach of super-resolution optical microscopy, which allows cellular imaging with so far unprecedented unlimited spatial resolution.…Read article
Super-resolution Microscopy and the Third Dimension
Optical imaging devices have a finite depth of field and diffraction limited resolution. The depth of field problem was tackled first with confocal microscopes, diffraction unlimited resolution is…Read article
STED Microscopy: A New Chapter in Light Imaging
When Stefan Hell invented a technique that pushed beyond the diffraction limit of light for the first time, a new field of nanoscale imaging was born. Now laboratories around the world are using …Read article
Watching Molecule Movements in Live Cells
The newly developed STED-RICS microscopy method records rapid movements of molecules in live samples. By combining raster image correlation spectroscopy (RICS) with STED fluorescence microscopy,…Read article
New Labeling Tools Can Help to Realize the Full Potential of Super-Resolution Microscopy
Since super-resolution microscopy techniques revolutionized the concept of light microscopy by overcoming the physical diffraction limit, STED microscopy and other super-resolution techniques have…Read article
Abstracts of the 3rd European Super-Resolution User-Club Meeting
The 3rd meeting of the Leica Super-Resolution User Club was held from June 17th to 19th, 2013 in collaboration with Alberto Diaspro and the Italian Institute of Technology (IIT) in Genoa. Confocal and…Read articleSTED Nanoscopy with Time-Gated Detection: Theoretical and Experimental Aspects
In a stimulated emission depletion (STED) microscope the region in which fluorescence markers can emit spontaneously shrinks with continued STED beam action after a singular excitation event. This…Read article
STED and GSDIM: Diffraction Unlimited Resolution for all Types of Fluorescence Imaging
This article gives an overview of two different types of superresolution techniques. Stimulated emission depletion (STED) microscopy is a versatile and fast method that is based on point scanning…Read article
Gates Open for Improved Confocal Fluorescence and Super-Resolution STED
True confocal microscope systems feature single-point illumination and single-point detection. The method is called "optical sectioning" since the generated image contains only information from the…Read article
Mitochondrial DNA Molecules are Packaged Individually
At the Max Planck Institute for Biology of Ageing in Cologne founded in 2008, three departments are working on the molecular, physiological and evolutionary mechanisms of the ageing of cells, tissue…Read article
Abstracts of the 2nd European Super-Resolution User-Club Meeting
The 2nd meeting of the Leica Super-resolution User club was held from September 25 to 27, 2012 in collaboration with the Science for Life Laboratory at the Karolinska Institute, Stockholm, Sweden. …Read article
Visualization of the Natural Killer Cell Immune Synapse by Super-Resolution Nanoscopy
Natural killer (NK) cells are innate immune effectors that recognize and kill virally infected and tumorigenic cells. Central to their effector function is the formation of an immunological synapse…Read article
Sharp Live Images from the Mouse Brain
To explore the most intricate structures of the brain in order to decipher how it functions – Stefan Hell’s team of researchers at the Max Planck Institute for Biophysical Chemistry in Göttingen has…Read article
Dual Channel STED Nanoscopy of Lytic Granules on Actin Filaments in Natural Killer Cells
Natural killer (NK) cells are innate immune effectors that eliminate diseased and tumorigenic targets through the directed secretion of specialized secretory lysosomes, termed lytic granules. This…Read article
Videos on STED Microscopy and its Inventor Stefan Hell
Here we present an collection of videos telling the story of the invention of nanometer-scale microscopy by Stefan Hell and explaining the basic principle of STED microscopy.Read article
Subcellular Localization of AKT and Tubulin using Super-Resolution Microscopy
Stimulated Emission Depletion microscopy, or STED nanoscopy, is a technique that uses the non-linear de-excitation of fluorescent dyes to overcome the resolution limit imposed by diffraction…Read article
Two-color STED Microscopy of Living Synapses using a Single Laser-beam Pair
The advent of superresolution microscopy has opened up new research opportunities into dynamic processes at the nanoscale inside living biological specimens. This is particularly true for synapses,…Read article
Modern Fluorescent Proteins and their Biological Applications
Here we present two review articles on fluorescent proteins and their biological applications. These first article reviews our current knowledge of blue, green, and red chromophore formation in…Read article
