Confocal Microscopy
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Studying early phase development of zebrafish embryos
MicaCam is where life science researchers come together live to chat, interact, and make discoveries together. Share your questions and engage during the livestream. Join us, and your life science…Read article
Cardiomyocyte Proliferation Upon Heart Injury in Zebrafish
This edition of MicaCam focuses on the study of the zebrafish (Danio rerio) whose heart cells can fully regenerate after injury.Read article
How Does The Cytoskeleton Transport Molecules?
See how 3D cysts derived from MDCK cells help scientists understand how proteins are transported and recycled in tissues and the role of the cytoskeleton in this transport.Read article
How To Get Multi Label Experiment Data With Full Spatiotemporal Correlation
The first edition of MicaCam focuses on the special challenges of live cell experiments. Our hosts Lynne Turnbull and Oliver Schlicker use the example of studying the mitochondrial activity of live…Read article
Gentle and Flexible 3D Imaging of Biological and Cleared Samples
For 3D imaging of biological samples, light sheet microscopy is a powerful and sensitive tool allowing you to measure (sub)cellular dynamics over long periods of time. Meanwhile, confocal microscopy,…Read article
Find Relevant Specimen Details from Fast Overviews
Switch from searching image by image to seeing the full overview of samples quickly and identifying the important specimen details instantly with confocal microscopy. Use that knowledge to set up…Read article
How to Target Fluorescent Structures in 3D for Cryo-FIB Milling
This article describes the major steps of the cryo-electron tomography workflow including super-resolution cryo-confocal microscopy. We describe how subcellular structures can be precisely located in…Read article
Precise 3D Targeting for EM Imaging - Access What Matters
Find out how the seamless cryo-electron tomography workflow Coral Cryo uses confocal super resolution to target your structure of interest more precisely.Read article
The Potential of Coherent Raman Scattering Microscopy at a Glance
Coherent Raman scattering microscopy (CRS) is a powerful approach for label-free, chemically specific imaging. It is based on the characteristic intrinsic vibrational contrast of molecules in the…Read article
3D Imaging of Tissues and Specimens
3D imaging enables thick tissue specimens and 3D cell cultures to be characterized intact, enabling scientists to visualize natural biological interactions. However, it is challenging to screen thick…Read article
Simplifying Complex Fluorescence Multiwell Plate Assays
Apoptosis, or programmed cell death, occurs during organism embryo development to eliminate unwanted cells and during healing in adults to rid the body of damaged cells and help prevent cancer.…Read article
Making Long-term Time-lapse Microscopy More Efficient
When doing time-lapse microscopy experiments with spheroids, there are certain challenges which can arise. As the experiments can last for several days, prolonged sample survival must be achieved…Read article
Structural HIV-1 Env Trimers Dynamics on Living T Cells
In this article, we focus on the molecular mechanism of the Env protein as it interacts with T cell, the primary target of HIV-1 [Carlon-Andres I et al 2021]. Multiphoton and fluorescence lifetime…Read article
Live-Cell Imaging Techniques
The understanding of complex and/or fast cellular dynamics is an important step for exploring biological processes. Therefore, today’s life science research is increasingly focused on dynamic…Read article
Imaging of Anti-Cancer Drug Uptake in Spheroids using DLS
Spheroid 3D cell culture models mimic the physiology and functions of living tissues making them a useful tool to study tumor morphology and screen anti-cancer drugs. The drug AZD2014 is a recognized…Read article
STELLARIS Contributes to Understanding COVID-19 Infection
What are the structural and functional consequences of a SARS-CoV-2 infection? To answer this important question, the authors used 3D cultures that mimic an airway epithelium and used different…Read article
Benefits of Combining STED and Lifetime
In this interview, Professor Alberto Diaspro talks about the advantages of the White Light Laser and the TauSTED capabilities of STELLARIS 8 STED. He speaks about his experience with the confocal…Read article
Spectroscopic Evaluation of Red Blood Cells
Hemoglobinopathies are a major healthcare problem. This study presents a possible diagnostic tool for thalassemia which is based on confocal spectroscopy. This approach exploits spectral detection and…Read article