Confocal Microscopy
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Evolutionary Morphology: Rostock Scientists Clarify Phylogenetic Relationships within the Most Diverse Taxon, Arthropoda
With over a million described species arthropods, which include spiders, millipedes, crustaceans and insects, are the most diverse taxon on earth. The total number of species of these segmented…Read article
The Relationship Between Membrane Potential and Calcium Dynamics in Glucose-Stimulated Beta Cell Syncytium in Acute Mouse Pancreas Tissue Slices
Oscillatory electrical activity is regarded as a hallmark of the pancreatic beta cell glucose-dependent excitability pattern. Electrophysiologically recorded membrane potential oscillations in beta…Read article
Euro-BioImaging – the pan-European Open Access Research Infrastructure Project for Biological and Medical Imaging Technologies
Euro‐BioImaging is a large‐scale pan‐European research infrastructure project on the European Strategy Forum on Research Infrastructures (ESFRI) Roadmap. Its mission is to build a distributed imaging…Read article
Acousto Optics in True Confocal Spectral Microscope Systems
Acousto-optical elements have successfully replaced planar filters in many positions. The white confocal, regarded as the fully spectrally tunable confocal microscope, was not possible without this…Read article
Nobel Prize 2013 in Physiology or Medicine for Discoveries of the Machinery Regulating Vesicle Traffic
On October 7th 2013, The Nobel Assembly at Karolinska Institutet has decided to award The Nobel Prize in Physiology or Medicine 2012 jointly to James E. Rothman, Randy W. Schekman and Thomas C. Südhof…Read article
A Flip of the Mitotic Spindle has Disastrous Consequences for Epithelial Cells
Constructing a body is like building a house – if you compromise structural integrity, the edifice can collapse. Nowhere is that clearer on a cellular level than in the case of epithelial sheets,…Read article
From Dynamic Live Cell Imaging to 3D Ultrastructure
To correlate dynamic events in adherent cells with both ultrastructural and 3D information, we developed a method for cultured cells that combines confocal time-lapse images of GFP-tagged proteins…Read article
Handbook of Optical Filters for Fluorescence Microscopy
Fluorescence microscopy and other light-based applications require optical filters that have demanding spectral and physical characteristics. Often, these characteristics are application-specific and…Read article
A Precise and Rapid Mapping Protocol for Correlative Light and Electron Microscopy of small invertebrate organisms
CLEM (correlative live cell and electronmicroscopy) seeks to bridge the data acquired with different imaging strategies, typically between light microscopy and electron microscopy. It has been…Read article
Spectral Detection – How to Define the Spectral Bands that Collect Probe-specific Emission
To specifically collect emission from multiple probes, the light is first separated spatially and then passes through a device that defines a spectral band. Classically, this is a common glass-based…Read article
Organ Regeneration: An Unlikely Fish Tale
Spectacular discoveries in cardiac tissue regeneration are rapidly moving researchers closer to the goal of harnessing regenerative techniques to repair the human heart. Only eleven years ago, Dr.…Read article
Pinhole Geometry: Four Corners are Perfect
Square and hexagonal pinholes provide identical image signal levels, if the geometries are compared in a sensible manner. The amount of light passing the pinhole depends on the area of that aperture,…Read article
Step by Step Guide for FRAP Experiments
Fluorescence Recovery After Photobleaching (FRAP) has been considered the most widely applied method for observing translational diffusion processes of macromolecules. The resulting information can be…Read article
Automated Digital 3D Topography Measurement with the Leica DCM 3D Dual Core Measuring Microscope
The precise measurement of surface structures and topography is the key thing during the production, control and development in many sectors of industry or research. Because very often there is not…Read article
The White Confocal – Spectral Gaps Closed
This article summarizes the development and differences in design and functionality of confocal technology as far as spectral properties are concerned, from classical filter-based excitation and…Read article
The White Confocal
Modern biomedical research is currently dominated by imaging and measuring with optical microscopes. One branch of the microscopy technology is confocal microscopy. For correlation purposes,…Read article
Molecular Developmental Biology: Norwegian Marine Research Scientists Solve the Mysteries of Evolution
The human nervous system is an infinitely complex network consisting of some 100 billions of neurons. It is the result of many-faceted evolutionary processes spanning millions of years which, like the…Read article
Gates Open for Improved Confocal Fluorescence and Super-Resolution STED
True confocal microscope systems feature single-point illumination and single-point detection. The method is called "optical sectioning" since the generated image contains only information from the…Read article