Confocal Microscopy
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Detailed Morphological Characterisation of Hendra Virus Infection of Different Cell Types Using Super-Resolution and Conventional Imaging
Hendra virus (HeV) is a pleomorphic virus belonging to the Paramyxovirus family. Our long-term aim is to understand the process of assembly of HeV virions. As a first step, we sought to determine the…Read article
Confocal and Digital Light Sheet Imaging
Optical imaging instrumentation can magnify tiny objects, zoom in on distant stars and reveal details that are invisible to the naked eye. But it notoriously suffers from an annoying problem: the…Read article
Venturing into Uncharted Dimensions – the Fascination of Future Technologies
Beyond the confines of the school curriculum, the young talent promotion program Initiative Junge Forscherinnen und Forscher e.V. (Initiative for Young Researchers) from Würzburg, Bavaria, has set…Read article
Third Harmonic Generation Microscopy – Label-Free 3D-Tissue Imaging and Blood Flow Characterization
THG microscopy as special variants of multiphoton microscopy. Third Harmonic Generation (THG) microscopy is a non-fluorescent multi-photon technique that combines the advantages of label-free…Read article
Interview with Dr. Gertrude Bunt and Prof. Fred S. Wouters on the FOM 2015
Only a few days to go before the start of Focus on Microscopy 2015 in Göttingen, Germany. This year’s FOM is being organized by Dr. Gertrude Bunt and Prof. Dr. Fred S. Wouters from the University…Read article
Customized Patterned Substrates for Highly Versatile Correlative Light-Scanning Electron Microscopy
Correlative light electron microscopy (CLEM) combines the advantages of light and electron microscopy, thus making it possible to follow dynamic events in living cells at nanometre resolution. Various…Read article
Video: High Speed Scanning – With two Scanners in one System
High speed scanning is necessary to image rapidly changing biological processes. With traditional scanning techniques, imaging speed is limited by the number of fluorophores in a specimen. And, rapid…Read article
Influence of Tissue and Plant Species in the Trafficking of a Recombinant Protein in Plant Cells
The development of recombinant DNA technology has allowed the use of plants for the production of biopharmaceuticals. In contrast to other production platforms, plants are unexpensive, easy to scale…Read article
"Leica is always flexible and dynamic" - Interview with Audrey Salles, Pasteur Institute, Paris
Audrey Salles is a specialist for confocal and super-resolution microscopy at Pasteur Institute, Imagopole, PFID, Paris, France. Her research interests are cytokine signaling and skeleton organization…Read article
Influencing Factors and Applicability of the Viability EMA-qPCR for a Detection and Quantification of Campylobacter Cells from Water Samples
In recent years, increasing numbers of human campylobacteriosis cases caused by contaminated water have been reported. As the culture-based detection of Campylobacter is time consuming and can yield…Read article
Axon Injury and Regeneration in the Adult Drosophila
Neural regeneration is a fascinating process with profound impact on human health, such that defining biological and genetic pathways is of interest. Here we describe an in vivo preparation for…Read article
Deeper Insights in Transparent Animals
CLARITY clearing derivatives for multiphoton microscopy. Transparent organisms help us to identify spatial arrangements and connections of cells and tissues, especially neuronal circuits can easily…Read article
Smart Control for Resonant Galvo Scanners
High time-resolution confocal microscopy (HTRCLSM) requires fast scanning devices. Whereas non-resonant galvo scanners allow full position control, but only at slow speed, resonant scanners allow…Read article
Characterization of Thin Films Using High Definition Confocal Microscopy
Thin film characterization technologies are in high demand, given the wide-spread use of coatings in all engineering and science fields. The properties of thin films can vary dramatically, i.e.…Read article
Imaging Pheromone Sensing in a Mouse Vomeronasal Acute Tissue Slice Preparation
In mice, the ability to detect pheromones is principally mediated by the vomeronasal organ (VNO). Here, an acute tissue slice preparation of VNO for performing calcium imaging is described. This…Read article
Genomic Survey, Gene Expression Analysis and Structural Modeling Suggest Diverse Roles of DNA Methyltransferases in Legumes
DNA methylation plays a crucial role in development through inheritable gene silencing. Plants possess three types of DNA methyltransferases (MTases), namely Methyltransferase (MET), Chromomethylase…Read article
Glucose-Stimulated Calcium Dynamics in Islets of Langerhans in Acute Mouse Pancreas Tissue Slices
In endocrine cells within islets of Langerhans calcium ions couple cell stimulation to hormone secretion. Since the advent of modern fluorimetry, numerous in vitro studies employing primarily isolated…Read article
The Effects of Peri-implantitis Decontamination Treatments on the Surface Roughness and Chemistry of a Titanium Alloy Used for Dental Implants: Implications for Bone Reintegration
Titanium (Ti) alloys are biocompatible materials which are often used for human implants, especially in dentistry. For this study, different types of odontological (dental) treatments for…Read article