Science Lab

Learn. Share. Contribute. The knowledge portal of Leica Microsystems. Find scientific research and teaching material on the subject of microscopy. The portal supports beginners, experienced practitioners and scientists alike in their everyday work and experiments. Explore interactive tutorials and application notes, discover the basics of microscopy as well as high-end technologies. Become part of the Science Lab community and share your expertise.

U2OS cells transfected with an Mx1-GFP plasmid (signal enhanced using Alexa Fluor 488-conjugared anti-GFP antibody) and co-stained for nuclear DNA (Hoechst 33342), microtubules (Alexa 555) and F-actin (ATTO 643). Image was captured on Mateo FL.

Microscopy and AI Solutions for 2D Cell Culture

This eBook explores the integration of microscopy and AI technologies in 2D cell culture workflows. It highlights how traditional imaging methods—such as brightfield, phase contrast, and…

Radially grown sugar crystals which have been imaged with a Leica microscope using circular polarized light.

Polarizing Microscope Image Gallery

How polarization microscope images can be used for analysis is shown in this gallery. Polarized light microscopy (also known as polarizing microscopy) is an important method for different fields and…

Artificial Intelligence (AI) segmentation used in conjunction with LMD to increase discovery throughput.

Biomarker Discovery with Laser Microdissection

Explore the potential of spatial proteomics workflows, such as Deep Visual Proteomics (DVP), to decipher pathology mechanisms and uncover druggable targets. Altered protein expression, abundance, or…

Image of roundworm C. elegans acquired with a M205 FA fluorescence automated stereo microscope in combination with Rottermann contrast. Areas labelled with mCherry are seen as reddish purple.

A Guide to C. elegans Research – Working with Nematodes

Efficient microscopy techniques for C. elegans research are outlined in this guide. As a widely used model organism with about 70% gene homology to humans, the nematode Caenorhabditis elegans (also…

Optic nerve regeneration in the Xenopus laevis tadpole.

A Novel Laser-Based Method for Studying Optic Nerve Regeneration

Optic nerve regeneration is a major challenge in neurobiology due to the limited self-repair capacity of the mammalian central nervous system (CNS) and the inconsistency of traditional injury models.…

Mouse brain slice which was immunostained with GFAP-A647 and imaged using a THUNDER Imager Tissue. Courtesy of H. Xu, University of Pennsylvania, Philadelphia, USA.

A Guide to Neuroscience Research

Neuroscience often requires investigating challenging specimens to better understand the nervous system and disorders. Leica microscopes helps neuroscientists obtain insights into neuronal functions.

A Guide to Zebrafish Research

To obtain optimal results while doing zebrafish research, especially during screening, sorting, handling, and imaging, seeing the fine details and structures is important. They help researchers make…

Zebrafish-embryo image captured using a THUNDER Imager Tissue and live instant computational clearing.

Improving Zebrafish-Embryo Screening with Fast, High-Contrast Imaging

Discover from this article how screening of transgenic zebrafish embryos is boosted with high-speed, high-contrast imaging using the DM6 B microscope, ensuring accurate targeting for developmental…

Pancreatic Ductal Adenocarcinoma with 11 Apoptosis biomarkers shown – BAK, BAX, BCL2, BCLXL, Caspase9, CIAP1, NaKATPase, PCK26, SMAC, Vimentin, and XIAP.

Transforming Research with Spatial Proteomics Workflows

Spatial Proteomics, Nature Methods 2024 Method of the Year, is driving research advancements in cancer, immunology, and beyond. By combining positional data with high throughput imaging of proteins in…