Science Lab

Learn. Share. Contribute. The knowledge portal of Leica Microsystems. Find scientific research and teaching material on the subject of microscopy. The portal supports beginners, experienced practitioners and scientists alike in their everyday work and experiments. Explore interactive tutorials and application notes, discover the basics of microscopy as well as high-end technologies. Become part of the Science Lab community and share your expertise.

Single cells collected via laser microdissection as part of the Deep Visual Proteomics workflow.

AI meets Deep Visual Proteomics (DVP) to Advance Disease Research

In this webinar, Dr. Andreas Mund introduces Deep Visual Proteomics (DVP) – a cutting-edge platform that integrates AI-powered tissue modeling with spatially resolved, untargeted proteomics. He…

U2OS cells transfected with an Mx1-GFP plasmid (signal enhanced using Alexa Fluor 488-conjugared anti-GFP antibody) and co-stained for nuclear DNA (Hoechst 33342), microtubules (Alexa 555) and F-actin (ATTO 643). Image was captured on Mateo FL.

Microscopy and AI Solutions for 2D Cell Culture

This eBook explores the integration of microscopy and AI technologies in 2D cell culture workflows. It highlights how traditional imaging methods—such as brightfield, phase contrast, and…

Cell DIVE multiplexed image of FFPE tissue section from human invasive ductal carcinoma (IDC)

AI-Powered Hi-Plex Spatial Analysis Tools for Breast Cancer Research

Breast cancer (BC) is the leading cause of cancer-related deaths in women. Investigating the tumor microenvironment (TME) is crucial to elucidate the mechanisms of tumor progression. Systematic…

67-hour, multi-position time-lapse of mouse intestinal organoids expressing the cell cycle reporter FUCCI2 (hGem-mVenus and hCdt1-mCherry).

Focus on Long-Term Imaging in 3D with Light Sheet Microscopy

Long-term 3D imaging reveals how complex multicellular systems grow and develop and how cells move and interact over time, unlocking critical insights into development, disease, and regeneration.…

Final Segmentation of organelles in Trichomonas species. Magenta – costa, light blue – hydrogenosomes, turquoise – ER, red – vacuoles, yellow – axostyle, green – Golgi apparatus. Sample courtesy of Isabelle Guerin-Bonne, Low Kay En, Electron Microscopy Unit, Yong Loo Lin School of Medicine, National University of Singapore. Scale bar: 1 µm.

Volume EM and AI Image Analysis

The article outlines a detailed workflow for studying biological tissues in three dimensions using volume-scanning electron microscopy (volume-SEM) combined with AI-assisted image analysis. The focus…

Dr. Nordmann in conversation with Dr. Falk Schlaudraff, Manager Product Management Uprights (widefield/compound) at Leica Microsystems.

How a Breakthrough in Spatial Proteomics Saved Lives

Toxic epidermal necrolysis (TEN) is a rare but devastating reaction to common medications like antibiotics or gout treatments. It begins innocuously, often as a rash, but can escalate rapidly into…

Fluorescence microscopy of sectioned tissue, showing the interface between the extensor digitorum longus muscle and the common peroneal nerve in the adult rat. Regenerative peripheral nerve interface (RPNI) at 2 weeks. Image acquired using Mica. Stained for nuclei (blue), neurofilaments (green) and S100B (red). Image courtesy of Dr. Aaron Lee, Department of Bioengineering (Lab of Dr. Rylie Green), Imperial College London.

How to Image Axon Regeneration in Deep Muscle Tissue

This study highlights Dr. Aaron Lee’s research on mapping nerve regeneration in muscle grafts post-amputation. Limb loss often leads to reduced quality of life, not only from tissue loss but also due…

5 hour time-lapse maximum intensity projection of a zebrafish embryo along the z-axis at 3 days post fertilization. Left: microglia cells. Right: bright field channel. Courtesy of Prof. Francesca Peri, University of Zurich, Switzerland.

Capturing Developmental Dynamics in 3D

This application note showcases how the Viventis Deep dual-view light sheet microscope was successfully used by researchers for exploring high-resolution, long-term imaging of 3D multicellular models…

These images illustrate the need for multiple z-slices to capture all gH2Ax foci in a given cell and get an accurate count.

Development and Derisking of CRISPR Therapies for Rare Diseases

This on-demand presentation by Dr. Fyodor Urnov and Dr. Sadik Kassim, originally delivered at ASGCT 2025, focused on a critical challenge in genetic medicine: how to scale CRISPR therapies from…