Science Lab

Learn. Share. Contribute. The knowledge portal of Leica Microsystems. Find scientific research and teaching material on the subject of microscopy. The portal supports beginners, experienced practitioners and scientists alike in their everyday work and experiments. Explore interactive tutorials and application notes, discover the basics of microscopy as well as high-end technologies. Become part of the Science Lab community and share your expertise.

3D rendering of a mouse beta cell with mitochondria (blue), insulin SGs (orange), microtubules (red), nucleus (yellow), and plasma membrane (transparent).

High-Pressure Freezing Protocols for Ultrastructural 3D EM

High pressure freezing (HPF) can help preserve hydrated cells and tissues close to their biological state at the moment of immobilization, supporting more reliable ultrastructural interpretation than…

Image of glass with primary corundum having numerous inclusions. It was taken with a microscope using polarization contrast.

Ensuring Glass Quality with the Polarization Microscopy Advantage

Glass is one of the oldest materials known. Today, it is used for many applications, e.g., optical instruments, windows, doors, solar panels, containers for food, beverages, and medicine, so strict…

Mouse fibroblasts where F-actin is labelled with FITC (green), tubulin with Cy5 (red), and nuclei with DAPI (blue). Image courtesy of Dr. Günter Giese, Max Planck Institute for Medical Research, Heidelberg, Germany.

Overview of Fluorescent Dyes in terms of Applications and Properties

An introduction to commonly used fluorescent dyes and an overview of their characteristics are given in this article. Fluorescence microscopy is used for the study of specific cellular components with…

Eliminating Electrostatic Interference in Laser Microdissection

Electrostatic charge in laser microdissection (LMD) causes two critical failures: samples stick to charged surfaces and are lost, or samples fly into adjacent wells and cause cross-contamination. We…

Region of a patterned wafer inspected using optical microscopy and automated and reproducible DIC (differential interference contrast). With DIC users are able to visualize small height differences on the wafer surface more easily.

6-Inch Wafer Inspection Microscope for Reliably Observing Small Height Differences

A 6-inch wafer inspection microscope with automated and reproducible DIC (differential interference contrast) imaging, no matter the skill level of users, is described in this article. Manufacturing…

A Larger 3D Area in Focus for Neurosurgical and Ophthalmic Microscopes

Neurosurgeons and ophthalmologists deal with delicate structures, deep or narrow cavities and tiny structures with vitally important functions. Seeing a clear, large 3D area of the surgical field in…

The infinite light path of a Leica microscope can be accessed between the light source and objective lens. This approach utilizing mirrors and beam splitters in the illumination path allows users to perform multiple applications with the microscope.

Infinity Optical Systems - From “Infinity Optics” to the Infinity Port

“Infinity Optics” is the concept of a light path with parallel rays between the objective and tube lens of a microscope [1]. Placing flat optical components into this “infinity space” which do not…

Quality assurance during production in a manufacturing plant.

Quality Assurance Improvement Across Industries

Precision is paramount. Imagine a pacemaker that fails mid-operation or a semiconductor flaw that causes a critical system crash. In industries, such as medical devices, electronics, and…

Aneurysm shown with GLOW800 AR fluorescence application. Image courtesy of Prof. Jacques Guyotat, Hôpital Neurologique Pierre Wertheimer, Lyon

How AR Fluorescence Imaging Supports Neurovascular Surgery

In this article, we explain how fluorescence imaging works in vascular neurosurgery and explain the benefits of the GLOW800 Augmented Reality fluorescence application.

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