Science Lab

Learn. Share. Contribute. The knowledge portal of Leica Microsystems. Find scientific research and teaching material on the subject of microscopy. The portal supports beginners, experienced practitioners and scientists alike in their everyday work and experiments. Explore interactive tutorials and application notes, discover the basics of microscopy as well as high-end technologies. Become part of the Science Lab community and share your expertise.

U2OS cells stained with Hoechst for nuclei (blue), MitoTracker green (Mitochondria structure, green) and TMRE (active mitochondria, magenta) and SiR for tubulin (red). Simultaneous acquisition of four channel large area overview using Spiral scan feature using the 10x/1.20 CS2 Water MotCORR objective.

How To Get Multi Label Experiment Data With Full Spatiotemporal Correlation

This video on demand focuses on the special challenges of live cell experiments. Our hosts Lynne Turnbull and Oliver Schlicker use the example of studying the mitochondrial activity of live cells.…

U2OS cells labelled with SiR Actin, TMRE, CellEvent™, and DAPI; 13-hour time-lapse imaging; apoptosis-inducer staurosporine

Simplifying Complex Fluorescence Multiwell Plate Assays

Apoptosis, or programmed cell death, occurs during organism embryo development to eliminate unwanted cells and during healing in adults to rid the body of damaged cells and help prevent cancer.…

Formation of 3D spheroids; Time lapse acquisition over 72 hours

Efficient Long-term Time-lapse Microscopy

When doing time-lapse microscopy experiments with spheroids, there are certain challenges which can arise. As the experiments can last for several days, prolonged sample survival must be achieved…

How to Choose a Microscope for Reconstructive Surgery

Plastic and reconstructive surgery requires excellent visualization to repair intricate and fine structures. Oncological reconstructive surgery procedures are among the most delicate, including breast…

Plastic reconstructive surgery with M530 OHX

Advances in Oncological Reconstructive Surgery

Decision making and patient care in oncological reconstructive surgery have considerably evolved in recent years. New surgical assistance technologies are helping surgeons push the boundaries of what…

Colon adenocarcinoma with 13 biomarkers shown

Hyperplex Cancer Tissue Analysis at Single Cell Level with Cell DIVE

The ability to study how lymphoma cell heterogeneity is influenced by the cells’ response to their microenvironment, especially at the mutational, transcriptomic, and protein levels. Protein…

Cellular microtubule network in a fibroblast cell.

How to Prepare your Specimen for Immunofluorescence Microscopy

Immunofluorescence (IF) is a powerful method for visualizing intracellular processes, conditions and structures. IF preparations can be analyzed by various microscopy techniques (e.g. CLSM,…

Single timepoint of a drosophilia embryo, 3D object detection

Examining Critical Developmental Events in High-Definition

Extended live cell imaging of embryo development requires a delicate balance between light exposure, temporal resolution and spatial resolution to maintain cells’ viability. Compromises between the…

How Industrial Applications Benefit from Fluorescence Microscopy

Watch this free webinar to know more about what you can do with fluorescence microscopy for industrial applications. We will cover a wide range of investigations where fluorescence contrast offers new…