Science Lab

Learn. Share. Contribute. The knowledge portal of Leica Microsystems. Find scientific research and teaching material on the subject of microscopy. The portal supports beginners, experienced practitioners and scientists alike in their everyday work and experiments. Explore interactive tutorials and application notes, discover the basics of microscopy as well as high-end technologies. Become part of the Science Lab community and share your expertise.

C. elegans Gonades - THUNDER Imager Adult hermaphrodit, Staining: blue - DAPI (Nucleus), green - SP56 (sperms), red - RME-2 (oocyte), mangenta - PGL-1 (RNA + protein granules) Image courtesy of Prof. Dr. Christian Eckmann, Martin Luther University, Halle, Germany

Physiology Image Gallery

Physiology is about the processes and functions within a living organism. Research in physiology focuses on the activities and functions of an organism’s organs, tissues, or cells, including the…

Tissue Image Gallery

Visual analysis of animal and human tissues is critical to understand complex diseases such as cancer or neurodegeneration. From basic immunohistochemistry to intravital imaging, confocal microscopy…

Virally labeled neurons (red) and astrocytes (green) in a cortical spheroid derived from human induced pluripotent stem cells. THUNDER Model Organism Imagerwith a 2x 0.15 NA objective at 3.4x zoomwas used to produce this 425 μm Z-stack (26 positions), which is presented here as an Extended Depth of Field(EDoF)projection.

Neuroscience Images

Neuroscience commonly uses microscopy to study the nervous system’s function and understand neurodegenerative diseases.

Dissecting Proteomic Heterogeneity of the Tumor Microenvironment

This lecture will highlight cutting edge applications in applying laser microdissection and microscaled quantitative proteomics and phosphoproteomics to uncover exquisite intra- and inter-tumor…

A Guide to Darkfield Microscopes

A darkfield microscope offers a way to view the structures of many types of biological specimens in greater contrast without the need of stains.

Pollen Flower - Taken with a 20x/0.8 objective, area of 6mm² with a depth of 100μm. 15 stitched tiles with 4 colors (DAPI/GFP/TRITC/Cy5) - a total of 13020 images. Video courtesy of James Marr, Leica Microsystems, USA

Developmental Biology Image Gallery

Developmental biology explores the development of complex organisms from the embryo to adulthood to understand in detail the origins of disease. This category of the gallery shows images about…

Kinetochore Assembly during Mitosis with TauSTED on 3D

Three-dimensional organization of the mitotic spindle together with the distribution of CENP-C and BUB1 based on TauSTED with multiple STED lines (592, 660 and 775 nm) can provide insights…

How to Quantify Changes in the Metabolic Status of Single Cells

Metabolic imaging based on fluorescence lifetime provides insights into the metabolic dynamics of cells, but its use has been limited as expertise in advanced microscopy techniques was needed. Now,…

Putting Dynamic Live Cell Data into the Ultrastructural Context

With workflow Coral Life, searching for a needle in the haystack is a thing of the past. Take advantage of correlative light and electron microscopy to identify directly the right cell at the right…