Science Lab

Learn. Share. Contribute. The knowledge portal of Leica Microsystems. Find scientific research and teaching material on the subject of microscopy. The portal supports beginners, experienced practitioners and scientists alike in their everyday work and experiments. Explore interactive tutorials and application notes, discover the basics of microscopy as well as high-end technologies. Become part of the Science Lab community and share your expertise.

Transverse histological cut of a rabbit tongue. 50 Mpixels images (2326 µm x 1739 µm) in 14 x 18 tiles. Lifetime gives an additional contrast that allows to differentiate different structures in histological stainings.

A Guide to Fluorescence Lifetime Imaging Microscopy (FLIM)

The fluorescence lifetime is a measure of how long a fluorophore remains on average in its excited state before returning to the ground state by emitting a fluorescence photon.

TauInteraction – Studying Molecular Interactions with TauSense

Fluorescence microscopy constitutes one of the pillars in life sciences and is a tool commonly used to unveil cellular structure and function. A key advantage of fluorescence microscopy resides in the…

Identification of distinct structures_roundworm_Ascaris_female

Find Relevant Specimen Details from Overviews

Switch from searching image by image to seeing the full overview of samples quickly and identifying the important specimen details instantly with confocal microscopy. Use that knowledge to set up…

Cellular microtubule network in a fibroblast cell.

How to Prepare your Specimen for Immunofluorescence Microscopy

Immunofluorescence (IF) is a powerful method for visualizing intracellular processes, conditions and structures. IF preparations can be analyzed by various microscopy techniques (e.g. CLSM,…

Fluorescent Dyes

A basic principle in fluorescence microscopy is the highly specific visualization of cellular components with the help of a fluorescent agent. This can be a fluorescent protein – for example GFP –…

Dynamic Signal Enhancement powered by Aivia: Truly simultaneous multicolor imaging of live cells (U2OS) in 3D

How Artificial Intelligence Enhances Confocal Imaging

In this article, we show how artificial intelligence (AI) can enhance your imaging experiments. Namely, how Dynamic Signal Enhancement powered by Aivia improves image quality while capturing the…

Spectroscopic Evaluation of Red Blood Cells

Hemoglobinopathies are a major healthcare problem. This study presents a possible diagnostic tool for thalassemia which is based on confocal spectroscopy. This approach exploits spectral detection and…

Visualizing Protein Degradation and Aggregation in the Living Cell

Our guest speaker, Prof Dr Eric Reits, presents his work on neurodegenerative disorders. Reits’ group are experts on the subject of Huntington’s disease and work towards identifying leads for…

Life Beyond the Pixels: Deep Learning Methods for Single Cell Analysis

Our guest speaker Prof Dr Peter Horvath presents his work on single cell-based large-scale microscopy experiments. This novel targeting approach includes the use of machine learning models and…