Science Lab

Science Lab

The knowledge portal of Leica Microsystems offers scientific research and teaching material on the subjects of microscopy. The content is designed to support beginners, experienced practitioners and scientists alike in their everyday work and experiments. Explore interactive tutorials and application notes, discover the basics of microscopy as well as high-end technologies – become part of the Science Lab community and share your expertise!

Latest Articles

Loading...

$STED and GSDIM: Diffraction Unlimited Resolution for all Types of Fluorescence Imaging$

STED and GSDIM: Diffraction Unlimited Resolution for all Types of Fluorescence Imaging

This article gives an overview of two different types of superresolution techniques. Stimulated emission depletion (STED) microscopy is a versatile…

Feb 28, 2013

Abstract
Loading...

The White Confocal – Spectral Gaps Closed

This article summarizes the development and differences in design and functionality of confocal technology as far as spectral properties are…

Feb 24, 2013

Abstract
Loading...

Light Sheet Fluorescence Microscopy: Beyond the Flatlands

Light Sheet Fluorescence Microscopy (LISH-M) is a true fluorescence optical sectioning technique, first described by Heinrich Siedentopf in 1902 under…

Dec 20, 2012

Abstract
Loading...

Developments in Multiphoton Excitation Microscopy

Basics, history, and applications of multiphoton microscopy. Honouring Goeppert-Mayer’s prediction of simultaneous two-photon absorption by an atom…

Oct 07, 2012

Abstract
Loading...

K-ATP Channels in Dopamine Substantia Nigra Neurons Control Bursting and Novelty-induced Exploration

Phasic activation of the dopamine (DA) midbrain system in response to unexpected reward or novelty is critical for adaptive behavioral strategies.…

Oct 04, 2012

Abstract
Loading...

Digital Camera Technologies for Scientific Bio-Imaging

This four-part series of articles published in Microscopy and Analysis covers the factors to consider in choosing a camera among CCD, EMCCD, and…

Jul 18, 2012

Abstract
Loading...

Order versus Disorder

In modern biomaterial design the generation of an environment mimicking some of the extracellular matrix features is envisaged to support molecular…

Apr 19, 2012

Abstract
Loading...

Sharp Live Images from the Mouse Brain

To explore the most intricate structures of the brain in order to decipher how it functions – Stefan Hell’s team of researchers at the Max Planck…

Mar 04, 2012

Abstract
Loading...

Dual Channel STED Nanoscopy of Lytic Granules on Actin Filaments in Natural Killer Cells

Natural killer (NK) cells are innate immune effectors that eliminate diseased and tumorigenic targets through the directed secretion of specialized…

Mar 01, 2012

Abstract
Loading...

Two-color STED Microscopy of Living Synapses using a Single Laser-beam Pair

The advent of superresolution microscopy has opened up new research opportunities into dynamic processes at the nanoscale inside living biological…

Nov 20, 2011

Abstract
Loading...

Quantitative Imaging in Cell Biology: Light Sheet Microscopy

This chapter introduces the concept of light sheet microscopy along with practical advice on how to design and build such an instrument. Selective…

Nov 20, 2011

Abstract
Loading...

Modern Fluorescent Proteins and their Biological Applications

Here we present two review articles on fluorescent proteins and their biological applications. These first article reviews our current knowledge of…

Nov 18, 2011

Abstract
Loading...

Focussed Ion Beam Milling and Scanning Electron Microscopy of Brain Tissue

This protocol describes how biological samples, like brain tissue, can be imaged in three dimensions using the focussed ion beam/scanning electron…

Jul 06, 2011

Abstract
Loading...

Light Sheet Fluorescence Microscopy - A Review

Light sheet fluorescence microscopy (LSFM) functions as a non-destructive microtome and microscope that uses a plane of light to optically section and…

Feb 20, 2011

Abstract
Loading...

ATP Changes the Fluorescence Lifetime of Cyan Fluorescent Protein via an Interaction with His148

This study employed fluorescence lifetime spectroscopy to investigate the influence of ATP (adenosine triphosphate) on the fluorescence intensities of…

Nov 05, 2010

Abstract
Loading...

A Guide to Super-Resolution Fluorescence Microscopy

For centuries, cell biology has been based on light microscopy and at the same time been limited by its optical resolution. However, several new…

Jul 18, 2010

Abstract
Loading...

Advanced Correlative Light/Electron Microscopy: Current Methods and New Developments Using Tokuyasu Cryosections

Microscopy is an essential tool for analysis of cellular structures and function. With the advent of new fluorescent probes and super-resolution light…

Dec 20, 2009

Abstract
Loading...

Selective Plane Illumination Microscopy Techniques in Developmental Biology

Selective plane illumination microscopy (SPIM) and other fluorescence microscopy techniques in which a focused sheet of light serves to illuminate the…

Jun 15, 2009

Abstract