Science Lab

The knowledge portal of Leica Microsystems offers scientific research and teaching material on the subjects of microscopy. The content is designed to support beginners, experienced practitioners and scientists alike in their everyday work and experiments. Explore interactive tutorials and application notes, discover the basics of microscopy as well as high-end technologies – become part of the Science Lab community and share your expertise!

Coherent Raman Scattering Microscopy Publication List

CRS (Coherent Raman Scattering) microscopy is an umbrella term for label-free methods that image biological structures by exploiting the characteristic, intrinsic vibrational contrast of their…

Formulated Product Characterization with SRS Microscopy

Creams, pastes, gels, emulsions, and tablets are ubiquitous across a wide range of manufacturing sectors from pharmaceuticals and consumer health products to agrochemicals and paint. To improve…

Tile scan of large intestine of an anasthesized mouse.

How to Quantify Label-free Blood Flow

The application note “Label-free Blood Flow Quantification” is a guide for data acquisition and analysis of blood flow using label-free third harmonic generation (THG) imaging.

Two-color SRS image of human pancreatic organoids showing proteins (red) and lipids (green).

Vibrational Spectroscopic Imaging in Cells, Tissues and Model Organisms

Here we present a webinar on the emerging applications of Stimulated Raman Scattering Microscopy, covering the following topics: Quantitative spectroscopic imaging of label-free or chemically tagged…

How to Prepare Samples for Stimulated Raman Scattering

Stimulated Raman Scattering (SRS) is an emerging microscopy technique that images the vibrations of chemical bonds within a specimen. It allows researchers to probe the endogenous chemistry or…

Step by Step Guide to the Molecular Basics of CARS Microscopy

CARS (Coherent Anti-Stokes Raman Scattering) microscopy is a dye-free method which images structures by displaying the characteristic intrinsic vibrational contrast of their molecules. The crucial…

Stimulated Raman Scattering Microscopy Probes Neurodegenerative Disease

Despite decades of research, the molecular mechanisms underlying some of the most severe neurodegenerative diseases, such as Alzheimer’s or Parkinson’s, remain poorly understood. The progression of…

CARS image of cellulose fibers. The fibers are visualized through the C–H vibrations of the polyglucan chains in cellulose.

CARS Microscopy: Imaging Characteristic Vibrational Contrast of Molecules

Coherent anti-Stokes Raman scattering (CARS) microscopy is a technique that generates images based on the vibrational signatures of molecules. This imaging methods does not require labeling, yet…

An Introduction to CARS Microscopy

CARS overcomes the drawbacks of conventional staining methods by the intrinsic characteristics of the method. CARS does not require labeling because it is highly specific to molecular compounds which…