Science Lab

Science Lab

Science Lab

The knowledge portal of Leica Microsystems offers scientific research and teaching material on the subjects of microscopy. The content is designed to support beginners, experienced practitioners and scientists alike in their everyday work and experiments. Explore interactive tutorials and application notes, discover the basics of microscopy as well as high-end technologies – become part of the Science Lab community and share your expertise!
Combining spectrally resolved detection and fluorescence lifetime multiplexing

Live-Cell Fluorescence Lifetime Multiplexing Using Organic Fluorophores

On-demand video: Imaging more subcellular targets by using fluorescence lifetime multiplexing combined with spectrally resolved detection.
Chicken-embryo cross section at the level of the midbrain showing neural crest cells and cadherin-6B molecules. Raw widefield data (top) and THUNDER image (bottom).

The Neural Crest (NC)

This article discusses how the study of neural crest (NC) development in chicken embryos is aided with haze-free imaging using a THUNDER Imager 3D Assay. Proper specification, migration, and…

Precise Spatial Proteomic Information in Tissues

Despite the availability of imaging-based and mass-spectrometry-based methods for spatial proteomics, a key challenge remains connecting images with single-cell-resolution protein abundance…

STED sheds insight on mitochondrial protein synthesis

In this paper the authors have adapted a click chemistry-based method coupled with stimulated emission depletion nanoscopy (STED) to address these questions reporting that, in human cells in culture,…

The Fundamentals and History of Fluorescence and Quantum Dots

At some point in your research and science career, you will no doubt come across fluorescence microscopy. This ubiquitous technique has transformed the way in which microscopists can image, tag and…

Real Time Observation of Neutrophil White Blood Cell Recruitment to Bacterial Infection In Vivo

The zebrafish (Danio rerio) is an emerging vertebrate model organism to study infection. The transparent larva comprises a fully functional innate immune system and enables live imaging of fluorescent…

What is Photomanipulation?

The term photomanipulation describes a wide range of techniques that enable the microscopist the transition from passive observer to instigator of events by offering a way of interacting with their…

Photoactivatable, photoconvertible, and photoswitchable Fluorescent Proteins

Fluorescent proteins (FPs) such as GFP, YFP or DsRed are powerful tools to visualize cellular components in living cells. Nevertheless, there are circumstances when classical FPs reach their limits.…

Video Talk by Roger Tsien: Fluorescent Protein Indicators

In this talk, Roger Tsien discusses how fluorescent proteins have been turned into indicators for a wide variety of biological molecules, including pH, ions, redox potential, and signaling molecules…
Scroll to top