Science Lab

The knowledge portal of Leica Microsystems offers scientific research and teaching material on the subjects of microscopy. The content is designed to support beginners, experienced practitioners and scientists alike in their everyday work and experiments. Explore interactive tutorials and application notes, discover the basics of microscopy as well as high-end technologies – become part of the Science Lab community and share your expertise!

Multi-color SRS image of a tri-cellular cancer spheroid

The Potential of Coherent Raman Scattering Microscopy at a Glance

Coherent Raman scattering microscopy (CRS) is a powerful approach for label-free, chemically specific imaging. It is based on the characteristic intrinsic vibrational contrast of molecules in the…

Two-color SRS image of human pancreatic organoids showing proteins (red) and lipids (green).

Vibrational Spectroscopic Imaging in Cells, Tissues and Model Organisms

Here we present a webinar on the emerging applications of Stimulated Raman Scattering Microscopy, covering the following topics: Quantitative spectroscopic imaging of label-free or chemically tagged…

How to Prepare Samples for Stimulated Raman Scattering

Stimulated Raman Scattering (SRS) is an emerging microscopy technique that images the vibrations of chemical bonds within a specimen. It allows researchers to probe the endogenous chemistry or…

Step by Step Guide to the Molecular Basics of CARS Microscopy

CARS (Coherent Anti-Stokes Raman Scattering) microscopy is a dye-free method which images structures by displaying the characteristic intrinsic vibrational contrast of their molecules. The crucial…

Stimulated Raman Scattering Microscopy Probes Neurodegenerative Disease

Despite decades of research, the molecular mechanisms underlying some of the most severe neurodegenerative diseases, such as Alzheimer’s or Parkinson’s, remain poorly understood. The progression of…

Coherent Raman Scattering (CRS) Microscopy: A vast and growing range of applications revealed

Leica Microsystems‘ Workshop on Coherent Raman Scattering (CRS) Microscopy attracted 30 enthusiastic participants from all over the world to beautiful Konstanz, Germany! The group enjoyed a number of…

CARS image of cellulose fibers. The fibers are visualized through the C–H vibrations of the polyglucan chains in cellulose.

CARS Microscopy: Imaging Characteristic Vibrational Contrast of Molecules

Coherent anti-Stokes Raman scattering (CARS) microscopy is a technique that generates images based on the vibrational signatures of molecules. This imaging methods does not require labeling, yet…

An Introduction to CARS Microscopy

CARS overcomes the drawbacks of conventional staining methods by the intrinsic characteristics of the method. CARS does not require labeling because it is highly specific to molecular compounds which…