Science Lab

The knowledge portal of Leica Microsystems offers scientific research and teaching material on the subjects of microscopy. The content is designed to support beginners, experienced practitioners and scientists alike in their everyday work and experiments. Explore interactive tutorials and application notes, discover the basics of microscopy as well as high-end technologies – become part of the Science Lab community and share your expertise!

Murine esophageal organoids (DAPI, Integrin26-AF 488, SOX2-AF568) imaged with the THUNDER Imager 3D Cell Culture. Courtesy of Dr. F.T. Arroso Martins, Tamere University, Finland.

How to Get Deeper Insights into your Organoid and Spheroid Models

In this eBook, learn about key considerations for imaging 3D cultures, such as organoids and spheroids, and discover microscopy solutions to shed new insights into dynamic processes in 3D real-time

Brain organoid section (DAPI) acquired using THUNDER Imager Live Cell. Image courtesy of Janina Kaspar and Irene Santisteban, Schäfer Lab, TUM.

Imaging Organoid Models to Investigate Brain Health

Imaging human brain organoid models to study the phenotypes of specialized brain cells called microglia, and the potential applications of these organoid models in health and disease.

Branched organoid growing in collagen where the Nuclei are labeled blue. To detect the mechanosignaling process, the YAP1 is labeled green.

Examining Developmental Processes In Cancer Organoids

Interview: Prof. Bausch and Dr. Pastucha, Technical University of Munich, discuss using microscopy to study development of organoids, stem cells, and other relevant disease models for biomedical…

The Role of Iron Metabolism in Cancer Progression

Iron metabolism plays a role in cancer development and progression, and modulates the immune response. Understanding how iron influences cancer and the immune system can aid the development of new…

Extended depth of field reconstruction image of a whole human pancreas islet showing fluorescence signals from insulin (green), glucagon (red), an IL17 cytokine (magenta), and nuclei (blue).

Understanding Better the Onset of Diabetes

This article shows how Interleukin-17 (IL-17) proinflammatory cytokine proteins in human pancreatic islets can be studied efficiently with a THUNDER Imager.

Harnessing Microfluidics to Maintain Cell Health During Live-Cell Imaging

VIDEO ON DEMAND - In this episode of MicaCam, we will use microfluidics to explore the effect of shear stress on cell morphology, examine the effect of nutrient replenishment on cellular growth during…

Effects of Clearing Media on Tissue Transparency and Shrinkage

This study comprehensively evaluates the effects of different clearing media on tissue transparency and shrinkage by comparing freshly dissected dipteran fly brains with their cleared equivalents.…

Fluorescence Lifetime-based Imaging Gallery

Confocal microscopy relies on the effective excitation of fluorescence probes and the efficient collection of photons emitted from the fluorescence process. One aspect of fluorescence is the emission…

Images of a brain organoid derived from iPSCs acquired with a THUNDER Imager 3D Cell Culture. The cells were infected with the pAAV-hSyn-EGFP and pLX-hGFAP-mCherry virus. The image is the 36th plane cropped out of a 53 plane Z-stack volume. Shown are both the A) raw widefield image and B) the same image after Large Volume Computation Clearing (LVCC). Neurons are labeled in green and astrocytes in red.

“Brains-In-A-Dish” from Induced Pluripotent Stem Cells (iPSCs)

This article discusses the benefits of using the THUNDER technology for imaging inside 3D human cortical brain organoids. These organoids are derived from human induced pluripotent stem cells (iPSCs)…