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How to Prepare Your Specimen for Immunofluorescence Microscopy
Immunofluorescence (IF) is a powerful method for visualizing intracellular processes, conditions and structures. IF preparations can be analyzed by…
Jan 10, 2022Story - Loading...
Fluorescent Dyes
A basic principle in fluorescence microscopy is the highly specific visualization of cellular components with the help of a fluorescent agent. This…
Jan 10, 2022Story - Loading...
The Guide to STED Sample Preparation
This guide is intended to help users optimize sample preparation for stimulated emission depletion (STED) nanoscopy, specifically when using the TCS…
Jul 22, 2019Tutorial - Loading...
Abstracts of the 7th European Super-Resolution User-Club Meeting
The 7th Super-Resolution User Club Meeting was held in collaboration with Prof Pavel Hozák , at the Institute of Molecular Genetics of the ASCR in…
Dec 17, 2017Story - Loading...
The Fundamentals and History of Fluorescence and Quantum Dots
At some point in your research and science career, you will no doubt come across fluorescence microscopy. This ubiquitous technique has transformed…
Nov 15, 2017Story - Loading...
Milestones in Incident Light Fluorescence Microscopy
Since the middle of the last century, fluorescence microscopy developed into a bio scientific tool with one of the biggest impacts on our…
Mar 06, 2017Story - Loading...
Testing the Münch Hypothesis of Long Distance Phloem Transport in Plants
Long distance transport in plants occurs in sieve tubes of the phloem. The pressure flow hypothesis introduced by Ernst Münch in 1930 describes a…
Feb 14, 2017Abstract - Loading...
Chronic Inflammation Under the Microscope
In the course of chronic inflammation certain body areas are recurrently inflamed. This goes along with many human diseases. With the help of…
Jan 09, 2017Story - Loading...
Multispectral Phloem-Mobile Probes: Properties and Applications
Using Arabidopsis (Arabidopsis thaliana) seedlings, we identified a range of small fluorescent probes that entered the translocation stream and were…
Nov 29, 2016Abstract - Loading...
Abstracts of the 6th European Super-Resolution User-Club Meeting
The 6th European Super-Resolution User Club Meeting was held in collaboration with Dr. Timo Zimmermann, CRG, and Dr. Pablo Loza-Alvarez, ICFO,…
Oct 18, 2016Story - Loading...
Video Talk by Roger Tsien: Fluorescent Protein Indicators
In this talk, Roger Tsien discusses how fluorescent proteins have been turned into indicators for a wide variety of biological molecules, including…
Aug 16, 2016Story - Loading...
Highly Selective Fluorescent and Colorimetric Probe for Live-cell Monitoring of Sulphide Based on Bioorthogonal Reaction
H2S is the third endogenously generated gaseous signaling compound and has also been known to involve a variety of physiological processes. To better…
Jul 29, 2016Abstract - Loading...
Video Talk by Roger Tsien: Fluorescent Proteins
Live cell imaging has been revolutionized by the discovery of the green fluorescent protein (GFP). This lecture covers the history of GFP, how it…
Oct 07, 2015Story - Loading...
Fluorescent Proteins Illuminate Cell Biology
Green fluorescent protein (GFP) isolated from the jellyfish Aequorea victoria and GFP-like fluorescent proteins from other animals have had an…
Sep 18, 2015Abstract - Loading...
STED Nanoscopy with Fluorescent Quantum Dots
The widely popular class of quantum-dot molecular labels could so far not be utilized as standard fluorescent probes in STED (stimulated emission…
Aug 13, 2015Abstract - Loading...
The Force of the Dark Side – Embedding Media for GSDIM Super-Resolution Localization Microscopy
Super-resolution microscopy such as Stimulated Emission Depletion (STED) and single-molecule based techniques rely on the same principle for breaking…
Jan 23, 2014Story - Loading...
Handbook of Optical Filters for Fluorescence Microscopy
Fluorescence microscopy and other light-based applications require optical filters that have demanding spectral and physical characteristics. Often,…
Aug 06, 2013Tutorial - Loading...
Step by Step Guide for FRAP Experiments
Fluorescence Recovery After Photobleaching (FRAP) has been considered the most widely applied method for observing translational diffusion processes…
May 17, 2013Tutorial