Science Lab

The knowledge portal of Leica Microsystems offers scientific research and teaching material on the subjects of microscopy. The content is designed to support beginners, experienced practitioners and scientists alike in their everyday work and experiments. Explore interactive tutorials and application notes, discover the basics of microscopy as well as high-end technologies – become part of the Science Lab community and share your expertise!

Microscopic images of GFP labelled vomeronasal sensory neuron axons forming glomeruli in the accessory olfactory bulb. Images acquired with a digital light sheet microscope from Leica Microsystems.

Investigating Axonal Pathfinding in the Accessory Olfactory System

Hernandez-Clavijo et. al. investigated the importance of TMEM16A, a calcium-activated chloride channel, for axonal pathfinding between the VNO and AOB and its effect on VSN density within the VNO.…

Wt1b-positive myocardial cell adopting an epicardial cell morphology.

Wt1 Genes Can Induce a Cardiomyocyte to Epicardial-like Cell Fate Transition

From this study, it was concluded that Wt1 plays a yet undescribed role for cardiomyocyte differentiation by repressing chromatin opening at specific genomic loci and that sustained ectopic expression…

Depth coding of endothelial cells in zebrafish eye. Courtesy of Basile Gurchenkov, Imaging Center of the IGBMC, Illkirch-Graffenstaden, France.

Gentle 3D Imaging of Biological Samples

For 3D imaging of biological samples, light sheet microscopy is a powerful and sensitive tool allowing you to measure (sub)cellular dynamics over long periods of time. Meanwhile, confocal microscopy,…

Dual color volume rendering of Drp1 oligomers (green) and mito OM (red) in a live U2OS cell

Multicolor 4D Super Resolution Light Sheet Microscopy

The AI Microscopy Symposium offers a unique forum for discussing the latest AI-based technologies and tools in the field of microscopy and biomedical imaging. In this scientific presentation, Yuxuan…

Imaging of Anti-Cancer Drug Uptake in Spheroids using DLS

Spheroid 3D cell culture models mimic the physiology and functions of living tissues making them a useful tool to study tumor morphology and screen anti-cancer drugs. The drug AZD2014 is a recognized…

Effects of Clearing Media on Tissue Transparency and Shrinkage

This study comprehensively evaluates the effects of different clearing media on tissue transparency and shrinkage by comparing freshly dissected dipteran fly brains with their cleared equivalents.…

Following Ca²⁺ Changes in Acutely Stimulated Spheroids

Spheroids are easy and cost-effective 3D cell cultures, useful for fundamental and applied research. They can bridge the gap between 2D and in vivo experiments and can be used for high-throughput…

Analyzing Structural and Functional Testicular Anatomy

Fleck, Kenzler et al. have shown that, in mice, muscle-like cells are located within the walls of seminiferous tubules. Using CLARITY and a Leica digital light-sheet (DLS) microscope, Fleck, Kenzler…

Fish and Chips Sheet into the Light

The zebrafish larval brain is small and contains about 1 million times less neurons than the human counterpart. The neural circuitry and the general bauplan of the vertebrate brain, however, is…