Science Lab

Science Lab

Science Lab

The knowledge portal of Leica Microsystems offers scientific research and teaching material on the subjects of microscopy. The content is designed to support beginners, experienced practitioners and scientists alike in their everyday work and experiments. Explore interactive tutorials and application notes, discover the basics of microscopy as well as high-end technologies – become part of the Science Lab community and share your expertise!

Factors to Consider When Selecting a Research Microscope

An optical microscope is often one of the central devices in a life-science research lab. It can be used for various applications which shed light on many scientific questions. Thereby the…

Eyepieces, Objectives and Optical Aberrations

For most microscope applications, there are generally only two sets of optics which are adjusted by the user, namely, the objectives and the eyepieces. Of course, this is assuming that the microscope…

Koehler Illumination: A Brief History and a Practical Set Up in Five Easy Steps

The technique of Koehler Illumination is one of the most important and fundamental techniques in achieving optimum imaging in any given light microscope set-up. Although it should be routinely used as…

Immersion Objectives: Using Oil, Glycerol, or Water to Overcome some of the Limits of Resolution

To examine specimens at high magnifications using the microscope, there are a number of factors which need to be taken into consideration. These include resolution, numerical aperture (NA), the…

Collecting Light: The Importance of Numerical Aperture in Microscopy

Numerical aperture (abbreviated as ‘NA’) is an important consideration when trying to distinguish detail in a specimen viewed down the microscope. NA is a number without units and is related to the…

Optimization of the Interplay of Optical Components for Aberration free Microscopy

Optical microscopes are used to magnify objects which are otherwise invisible for the human eye. For this purpose high quality optics is necessary to achieve appropriate resolution. However, besides…
Video Talk by Kurt Thorn: The Abbe Diffraction Experiment

Video Talk by Kurt Thorn: The Abbe Diffraction Experiment

This lecture describes the famous experiments of Ernst Abbe which showed how diffraction of light by a specimen (and interference with the illuminating light) gives rise to an image and how collection…

Microscope Resolution: Concepts, Factors and Calculation

In microscopy, the term ‘resolution’ is used to describe the ability of a microscope to distinguish detail. In other words, this is the minimum distance at which two distinct points of a specimen can…

Infinity Optical Systems

“Infinity Optics” refers to the concept of a beam path with parallel rays between the objective and the tube lens of a microscope. Flat optical components can be brought into this “Infinity Space”…
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