Science Lab

The knowledge portal of Leica Microsystems offers scientific research and teaching material on the subjects of microscopy. The content is designed to support beginners, experienced practitioners and scientists alike in their everyday work and experiments. Explore interactive tutorials and application notes, discover the basics of microscopy as well as high-end technologies – become part of the Science Lab community and share your expertise!

Five-color FLIM-STED with one depletion Laser

Webinar on five-color STED with a single depletion laser and fluorescence lifetime phasor separation.

A Versatile Palette of Fluorescent Probes

Researchers at the Max Planck Institute for Medical Research in Heidelberg have developed a general strategy to synthesize live-cell compatible fluorogenic probes, and the result are the new MaP (Max…

STED imaging of mitochondria revealed cristae dynamics during fusion. A comparison of confocal (left) and STED (right) imaging.

Mitochondrial dynamics quantitatively revealed by STED nanoscopy

Mitochondria are vital subcellular organelles, which can generate energy to support the entire lifecycle of biological cells. However, conventional fluorescence microscopy cannot provide a high enough…

Benefits of Combining STED and Lifetime

In this interview, Professor Alberto Diaspro talks about the advantages of the White Light Laser and the TauSTED capabilities of STELLARIS 8 STED. He speaks about his experience with the confocal…

Effects of Clearing Media on Tissue Transparency and Shrinkage

This study comprehensively evaluates the effects of different clearing media on tissue transparency and shrinkage by comparing freshly dissected dipteran fly brains with their cleared equivalents.…

Visualization of Submitochondrial Protein Distributions

By allowing visualization of submitochondrial protein distributions, fluorescence nanoscopy offers significant advantages for understanding cell death control. How cells manage, and control…

DNA Replication in Cancer Cells

DNA synthesis can be impeded by collisions between the DNA replication machinery and co-transcriptional R-loops leading to a major source of genomic instability in cancer cells. In this paper we…

Super-Resolution Microscopy Image Gallery

Due to the diffraction limit of light, traditional confocal microscopy cannot resolve structures below ~240 nm. Super-resolution microscopy techniques, such as STED, PALM or STORM or some…

Regulators of Actin Cytoskeletal Regulation and Cell Migration in Human NK Cells

Dr. Mace will describe new advances in our understanding of the regulation of human NK cell actin cytoskeletal remodeling in cell migration and immune synapse formation derived from confocal and…