Science Lab

The knowledge portal of Leica Microsystems offers scientific research and teaching material on the subjects of microscopy. The content is designed to support beginners, experienced practitioners and scientists alike in their everyday work and experiments. Explore interactive tutorials and application notes, discover the basics of microscopy as well as high-end technologies – become part of the Science Lab community and share your expertise!

Coherent Raman Scattering Microscopy Publication List

CRS (Coherent Raman Scattering) microscopy is an umbrella term for label-free methods that image biological structures by exploiting the characteristic, intrinsic vibrational contrast of their…

Intravital Microscopy of Cancer

Join our guest speaker Prof Dr Jacco van Rheenen, as he presents his work on the identity, behavior and fate of cells that drive the initiation and progression of cancer.

Tile scan of large intestine of an anasthesized mouse.

How to Quantify Label-free Blood Flow

The application note “Label-free Blood Flow Quantification” is a guide for data acquisition and analysis of blood flow using label-free third harmonic generation (THG) imaging.

Principles of Multiphoton Microscopy for Deep Tissue Imaging

Basics of multiphoton microscopy. This tutorial explains the principles of multiphoton microscopy for deep tissue imaging. Multiphoton microscopy uses excitation wavelengths in the infrared taking…

Step by Step Guide to the Molecular Basics of CARS Microscopy

CARS (Coherent Anti-Stokes Raman Scattering) microscopy is a dye-free method which images structures by displaying the characteristic intrinsic vibrational contrast of their molecules. The crucial…

DIVE Multiphoton Microscope Image Gallery

Today’s life science research focusses on complex biological processes, such as the causes of cancer and other human diseases. A deep look into tissues and living specimens is vital to understanding…

Which Sensor is the Best for Confocal Imaging?

The Hybrid Photodetectors (HyD) are! Why that is the case is explained in this short Science Lab article.

Multiphoton Microscopy – a Satisfied Wish List

The colorful picture shows colon tumor cells, fluorescently labelled and lineage traced from a multicolor tracer. The gray color codes for the second harmonic generation (SHG) signal from Collagen 1.…

Multiphoton microscopy of an unstained mouse skin section acquired using the 4Tune detector.

Mission Impossible Accomplished: Tunable Colors for Non-descanning Detection

Leica Microsystems’ 4Tune detector, the key component of the SP8 DIVE Deep In Vivo Explorer, provides spectrally tunable image recording with non-descanning detection. An innovative solution for…