Science Lab

Science Lab

Science Lab

The knowledge portal of Leica Microsystems offers scientific research and teaching material on the subjects of microscopy. The content is designed to support beginners, experienced practitioners and scientists alike in their everyday work and experiments. Explore interactive tutorials and application notes, discover the basics of microscopy as well as high-end technologies – become part of the Science Lab community and share your expertise!
Combining spectrally resolved detection and fluorescence lifetime multiplexing

Live-Cell Fluorescence Lifetime Multiplexing Using Organic Fluorophores

On-demand video: Imaging more subcellular targets by using fluorescence lifetime multiplexing combined with spectrally resolved detection.
Donor (D) and acceptor (A) molecule which participate in FRET (Förster resonance energy transfer).

What is FRET with FLIM (FLIM-FRET)?

This article explains the FLIM-FRET method which combines resonance energy transfer and fluorescence lifetime imaging to study protein-protein interactions.

Visualizing Protein-Protein Interactions by Non-Fitting and Easy FRET-FLIM Approaches

The Webinar with Dr. Sergi Padilla-Parra is about visualizing protein-protein interaction. He gives insight into non-fitting and easy FRET-FLIM approaches.

What is FLIM - Fluorescence Lifetime Imaging Microscopy?

FLIM exploits fluorescence lifetime which is a measure of how long a fluorescent molecule or fluorophore remains on average in its excited state before returning to the ground state by emitting a…
Transverse histological cut of a rabbit tongue. 50 Mpixels images (2326 µm x 1739 µm) in 14 x 18 tiles. Lifetime gives an additional contrast that allows to differentiate different structures in histological stainings.

A Guide to Fluorescence Lifetime Imaging Microscopy (FLIM)

The fluorescence lifetime is a measure of how long a fluorophore remains on average in its excited state before returning to the ground state by emitting a fluorescence photon.
Diagram showing the structural conformations found from the FLIM phasor data.

Structural HIV-1 Env Trimers Dynamics on Living T Cells

In this article, we focus on the molecular mechanism of the Env protein as it interacts with T cell, the primary target of HIV-1 [Carlon-Andres I et al 2021]. Multiphoton and fluorescence lifetime…

Fluorescence Lifetime-based Imaging Gallery

Confocal microscopy relies on the effective excitation of fluorescence probes and the efficient collection of photons emitted from the fluorescence process. One aspect of fluorescence is the emission…

Phasor FLIM Detects Metabolic Differences

The maintenance of normal blood glucose levels is defective in diabetes caused by dysfunction of the alpha and beta cells in islets. To understand these regularities, we used multiphoton phasor-FLIM…

Accessing the Metabolic Information of Stem Cells

Cancer often comes with changes in the metabolism of cells. Monitoring the metabolic state using fluorescence lifetime imaging microscopy (FLIM) is a good way to assess these changes. In some cases,…
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