
Science Lab
Science Lab
The knowledge portal of Leica Microsystems offers scientific research and teaching material on the subjects of microscopy. The content is designed to support beginners, experienced practitioners and scientists alike in their everyday work and experiments. Explore interactive tutorials and application notes, discover the basics of microscopy as well as high-end technologies – become part of the Science Lab community and share your expertise!
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FluoSync - a Fast & Gentle Method for Unmixing Multicolour Images
In this white paper, we focus on a fast and reliable method for obtaining high-quality multiplex images in fluorescence microscopy. FluoSync combines an existing method for hybrid unmixing with…
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Precise Spatial Proteomic Information in Tissues
Despite the availability of imaging-based and mass-spectrometry-based methods for spatial proteomics, a key challenge remains connecting images with single-cell-resolution protein abundance…
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Create New Options for Live Cell Imaging
The use of state-of-the-art AI systems is pushing image analysis into a new generation. Challenges like the conflict between imaging power and sample integrity are being overcome with THUNDER’s…
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How to Target Fluorescent Structures in 3D for Cryo-FIB Milling
This article describes the major steps of the cryo-electron tomography workflow including super-resolution cryo-confocal microscopy. We describe how subcellular structures can be precisely located in…
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How to Successfully Perform Live-cell CLEM
The Leica Nano workflow provides a streamlined live-cell CLEM solution for getting insight bout structural changes of cellular components over time. Besides the technical handling described in the…
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Overcoming Multiplexed Imaging Roadblocks
Whether a single slide or a batch of slides processed on multiple Cell DIVE imagers, every step carried out by the Cell DIVE Acquisition Software eliminates distortions and artifacts and seamlessly…
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A New World of Confocal Applications with the Next Generation White Light Lasers
As biological questions get more complex, there is an increasing need to study multiple events simultaneously in the same specimen. When preparing the specimen for imaging experiments, this need is…
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An Introduction to Computational Clearing
Many software packages include background subtraction algorithms to enhance the contrast of features in the image by reducing background noise. The most common methods used to remove background noise…
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Nanoscopy meets Lifetime: Introducing TauSTED
TauSTED combines the optical signals from STED and the photophysical information from the fluorescence lifetime at unprecedented speeds. This approach to STED uses phasor analysis in a novel way.…