Science Lab

Science Lab

Science Lab

The knowledge portal of Leica Microsystems offers scientific research and teaching material on the subjects of microscopy. The content is designed to support beginners, experienced practitioners and scientists alike in their everyday work and experiments. Explore interactive tutorials and application notes, discover the basics of microscopy as well as high-end technologies – become part of the Science Lab community and share your expertise!

What is FLIM - Fluorescence Lifetime Imaging Microscopy?

FLIM exploits fluorescence lifetime which is a measure of how long a fluorescent molecule or fluorophore remains on average in its excited state before returning to the ground state by emitting a…
Separation of cells based on their tracking status: A colourised binary mask of a time-lapse microscopy field of view of medium confluency with individual cells highlighted as survivors if they can be tracked since the initial movie frame (cyan), incomers if they migrated into the field of view throughout the movie (yellow) or mistracks if an error occurred in the automated trajectory reconstruction (red).

Tracking Single Cells Using Deep Learning

AI-based solutions continue to gain ground in the field of microscopy. From automated object classification to virtual staining, machine and deep learning technologies are powering scientific…
Analysis of anatomy and axon orientation of an adult mouse brain tissue with QLIPP

Learning the Cellular Architecture from its Optical Properties

In the last 3 years, microscopists have started to use "AI based" solutions for a wide range of applications, including image acquisition optimization (smart microscopy), object classification, image…

Phasor FLIM Detects Metabolic Differences

The maintenance of normal blood glucose levels is defective in diabetes caused by dysfunction of the alpha and beta cells in islets. To understand these regularities, we used multiphoton phasor-FLIM…

Accessing the Metabolic Information of Stem Cells

Cancer often comes with changes in the metabolism of cells. Monitoring the metabolic state using fluorescence lifetime imaging microscopy (FLIM) is a good way to assess these changes. In some cases,…

Label-free Differentiation of Microplastics

Phasor analysis for fluorescence lifetime imaging microscopy (FLIM) is a powerful and fitting-free tool that provides a 2D graphical assessment of pixel-accurate lifetime distributions in a sample…

A Quality Metric for the Systematic Evaluation of Clearing Protocols

3D multicellular spheroids are of interest for studying tumor behavior and evaluating the response of pharmacologically active agents, because they mimic the in vivo tumor environment better than…

How FLIM Microscopy helps to detect microplastic pollution

The use of autofluorescence in biological samples is a widely used method to gain detailed knowledge about systems or organisms. This property is not only found in biological systems, but also…

Imaging Intracellular Temperature using FLIM

This video shows the presentation of Dr. Kohki Okabe given at the 9th Congress of the Federation of Asian and Oceanian Physiological Society (FAOPS) held in Kobe, Japan on 30 March 2019. Dr. Okabe…
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