Science Lab

The knowledge portal of Leica Microsystems offers scientific research and teaching material on the subjects of microscopy. The content is designed to support beginners, experienced practitioners and scientists alike in their everyday work and experiments. Explore interactive tutorials and application notes, discover the basics of microscopy as well as high-end technologies – become part of the Science Lab community and share your expertise!

Studying Virus Replication with Fluorescence Microscopy

The results from research on SARS-CoV-2 virus replication kinetics, adaption capabilities, and cytopathology in Vero E6 cells, done with the help of fluorescence microscopy, are described in this…

Pancreatic Ductal Adenocarcinoma with 11 cancer immunity and progression biomarkers shown.

Mastering Multiplex Immunofluorescence: Techniques and Troubleshooting

This webinar combines the expertise to provide a comprehensive overview of multiplex immunofluorescence staining and imaging with practical tips.

Cell counts for each biomarker were divided by total number of cells to give a percentage of biomarker positive cells out of total cells for each biomarker.

Methods to Improve Reproducibility in Spatial Biology Research

Establish reproducibility results for a Cell DIVE multiplexed imaging study in cancer research using the BAB 200 automated system from ASLS and validated antibodies from CST

PDAC Multiplexed imaging of CST panels enables an examination of immune cell components in pancreatic ductal adenocarcinoma (IPDAC) tissue on a single slide.

Characterizing Tumor Environment to Reveal Insights and Spatial Resolution

Antibodies from Cell Signaling Technology are validated for use with the Cell DIVE multiplexing workflow and used to probe cell lineages in the tumor microenvironment

Pancreatic Ductal Adenocarcinoma with 5 biomarkers shown – SMA, PanCK PCK26, PanCK AE1, Vimentin, and Glut1.

Dig Deeper Into the Complexities of Pancreatic Cancer with Multiplex Imaging

Cell DIVE is an iterative staining workflow for multiplexed imaging that unveils biological pathways to dig deeper into the complexities of pancreatic cancer.

Complex Made Simple: Antibodies in Multiplexed Imaging

Build panels, plan studies, and get the most from precious reagents using this antibody multiplexing guide from Leica Microsystems

Raw widefield (left) and Computationally Cleared image (right) of mouse neuromuscular junctions acquired with a THUNDER Imager. Courtesy of A. Yung and M. Krasnow in California, USA.

Neural Basis of Breathing, Vocalization, and Diseases of the Larynx

This article shows how research on diseases of the larynx can be improved with a THUNDER Imager and Computational Clearing. Motor neurons in whole mounts of mouse laryngeal muscle are better…

Cell DIVE: Unraveling Pathways in Pancreatic Cancer

Unlock new insights into tumour-immune interactions with spatial biology. Join our webinar to learn how to label >60 biomarkers per tissue section and use advanced clustering and analysis techniques…

Pancreatic Ductal Adenocarcinoma with 11 cancer immunity and progression biomarkers shown –CD3, CD4, CD20, CD31, CD45, Collagen, HLA1, NaKATPase, PCK26, SMA1, and Vimentin.

Open Multiplexing: Transforming Tissue Research with Cell DIVE

Watch this webinar to discover how open multiplexing adapts to meet the needs of your multiplexing imaging study.