The Microscopy Knowledge Portal

Plant researchers Takumi Higaki and Hidenobu Mizuno have developed a framework to map leaf pavement cells in 4D using AI software with VR equipment.

What are the structural and functional consequences of a SARS-CoV-2 infection? To answer this important question, the authors used 3D cultures that…

In the present study, we show, in an in vivo longitudinal study, that the Slit-Robo pathway is critical for immunosuppressive myeloid cell…

In this paper the authors have adapted a click chemistry-based method coupled with stimulated emission depletion nanoscopy (STED) to address these…

Mitochondria are vital subcellular organelles, which can generate energy to support the entire lifecycle of biological cells. However, conventional…

Hemoglobinopathies are a major healthcare problem. This study presents a possible diagnostic tool for thalassemia which is based on confocal…

By allowing visualization of submitochondrial protein distributions, fluorescence nanoscopy offers significant advantages for understanding cell death…

DNA synthesis can be impeded by collisions between the DNA replication machinery and co-transcriptional R-loops leading to a major source of genomic…

The maintenance of normal blood glucose levels is defective in diabetes caused by dysfunction of the alpha and beta cells in islets. To understand…

Spheroids are easy and cost-effective 3D cell cultures, useful for fundamental and applied research. They can bridge the gap between 2D and in vivo…

Fleck, Kenzler et al. have shown that, in mice, muscle-like cells are located within the walls of seminiferous tubules. Using CLARITY and a Leica…

To follow cancer metastasis at the point of occurrence intravital multiphoton microscopy is the tool of choice. Intravital microscopy allows…

Cancer often comes with changes in the metabolism of cells. Monitoring the metabolic state using fluorescence lifetime imaging microscopy (FLIM) is a…

Three-dimensional organization of the mitotic spindle together with the distribution of CENP-C and BUB1 based on TauSTED with multiple STED lines…

TauSTED, with low photon budget and the fast detection scheme in our STELLARIS confocal platform, enables the tracking of highly dynamic processes in…

This article demonstrates that the stress-activated protein kinase pathway (SAPK) and its effector, MAPK Sty1, downregulates CAR assembly in the…

Phasor analysis for fluorescence lifetime imaging microscopy (FLIM) is a powerful and fitting-free tool that provides a 2D graphical assessment of…

In this article, a ratiometric fluorometric method for cytosolic calcium ion (Ca2+) measurement in cultured melanoma cells using Fura 2-AM cell…