The Microscopy Knowledge Portal
The knowledge portal of Leica Microsystems offers scientific research and teaching material on the subjects of microscopy. The content is designed to support beginners, experienced practitioners and scientists alike in their everyday work and experiments. Explore interactive tutorials and application notes, discover the basics of microscopy as well as high-end technologies – become part of the Science Lab community and share your expertise!
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Latest Articles
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Spectroscopic Evaluation of Red Blood Cells
Hemoglobinopathies are a major healthcare problem. This study presents a possible diagnostic tool for thalassemia which is based on confocal…
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Visualization of Submitochondrial Protein Distributions
By allowing visualization of submitochondrial protein distributions, fluorescence nanoscopy offers significant advantages for understanding cell death…
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DNA Replication in Cancer Cells
DNA synthesis can be impeded by collisions between the DNA replication machinery and co-transcriptional R-loops leading to a major source of genomic…
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Phasor FLIM Detects Metabolic Differences
The maintenance of normal blood glucose levels is defective in diabetes caused by dysfunction of the alpha and beta cells in islets. To understand…
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Following Ca²⁺ Changes in Acutely Stimulated Spheroids
Spheroids are easy and cost-effective 3D cell cultures, useful for fundamental and applied research. They can bridge the gap between 2D and in vivo…
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Analyzing Structural and Functional Testicular Anatomy
Fleck, Kenzler et al. have shown that, in mice, muscle-like cells are located within the walls of seminiferous tubules. Using CLARITY and a Leica…
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Gaining Insights into Colorectal Cancer Metastasis
To follow cancer metastasis at the point of occurrence intravital multiphoton microscopy is the tool of choice. Intravital microscopy allows…
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Accessing the Metabolic Information of Stem Cells
Cancer often comes with changes in the metabolism of cells. Monitoring the metabolic state using fluorescence lifetime imaging microscopy (FLIM) is a…
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Kinetochore Assembly during Mitosis with TauSTED on 3D
Three-dimensional organization of the mitotic spindle together with the distribution of CENP-C and BUB1 based on TauSTED with multiple STED lines…
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Fast 2 colour live cell dynamics with TauSTED gentle super-resolution
TauSTED, with low photon budget and the fast detection scheme in our STELLARIS confocal platform, enables the tracking of highly dynamic processes in…
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Fission Yeast Actomyosin Ring Integrity
This article demonstrates that the stress-activated protein kinase pathway (SAPK) and its effector, MAPK Sty1, downregulates CAR assembly in the…
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Label-free Differentiation of Microplastics
Phasor analysis for fluorescence lifetime imaging microscopy (FLIM) is a powerful and fitting-free tool that provides a 2D graphical assessment of…
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Cytosolic Calcium Ions in Melanoma Cancer Cells
In this article, a ratiometric fluorometric method for cytosolic calcium ion (Ca2+) measurement in cultured melanoma cells using Fura 2-AM cell…
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Fish and Chips Sheet into the Light
The zebrafish larval brain is small and contains about 1 million times less neurons than the human counterpart. The neural circuitry and the general…
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A Quality Metric for the Systematic Evaluation of Clearing Protocols
3D multicellular spheroids are of interest for studying tumor behavior and evaluating the response of pharmacologically active agents, because they…
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How FLIM Microscopy helps to detect microplastic pollution
The use of autofluorescence in biological samples is a widely used method to gain detailed knowledge about systems or organisms. This property is not…
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OCT-Analysis of a Clear Corneal Incision
The aim of this prospective study was to analyse the effect of lateral stromal hydration on the morphology of clear corneal incision architecture…
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Coronavirus Replication in Host Cells
In this study, a molecular pore complex, which spans both membranes of SARS-CoV-2/COVID-19 virus-induced cytosolic vesicles and likely plays a role in…
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- Otolaryngology (ENT)
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