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Total RNA Isolation after Laser-capture Microdissection of Human Cervical Squamous Epithelial Cells from Fresh Frozen Tissue
As most tissue specimens contain a mixture of different cell types including epithelial cells, stromal cells and immune cells, selection of the cells…
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Pathways to Optical STED Microscopy
STED nanoscopy has evolved to a highly versatile tool for the observation of the living cell, more and more finding its way into state-of-the-art…
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Customized Patterned Substrates for Highly Versatile Correlative Light-Scanning Electron Microscopy
Correlative light electron microscopy (CLEM) combines the advantages of light and electron microscopy, thus making it possible to follow dynamic…
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Super-resolution Imaging of the Natural Killer Cell Immunological Synapse on a Glass-supported Planar Lipid Bilayer
The glass-supported planar lipid bilayer system has been utilized in a variety of disciplines. One of the most useful applications of this technique…
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Multi-Images Deconvolution Improves Signal-to-Noise Ratio on Gated Stimulated Emission Depletion Microscopy
Time-gated detection, namely, only collecting the fluorescence photons after a time-delay from the excitation events, reduces complexity, cost, and…
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Nature Methods: Light-sheet Fluorescence Microscopy - Method of the Year 2014
Just about everyone who has examined fluorescent samples under the microscope is aware of the constant struggle to have enough signal to see the…
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Influencing Factors and Applicability of the Viability EMA-qPCR for a Detection and Quantification of Campylobacter Cells from Water Samples
In recent years, increasing numbers of human campylobacteriosis cases caused by contaminated water have been reported. As the culture-based detection…
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Axon Injury and Regeneration in the Adult Drosophila
Neural regeneration is a fascinating process with profound impact on human health, such that defining biological and genetic pathways is of interest.…
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Identification of Molecular Pathways Facilitating Glioma Cell Invasion In Situ
Gliomas are mostly incurable secondary to their diffuse infiltrative nature. Thus, specific therapeutic targeting of invasive glioma cells is an…
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Electron Tomography of Cryo-Immobilized Plant Tissue: A Novel Approach to Studying 3D Macromolecular Architecture of Mature Plant Cell Walls In Situ
Cost-effective production of lignocellulosic biofuel requires efficient breakdown of cell walls present in plant biomass to retrieve the wall…
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A New Probe for Super-Resolution Imaging of Membranes Elucidates Trafficking Pathways
The molecular composition of the organelles involved in membrane recycling is difficult to establish as a result of the absence of suitable labeling…
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Defect Transport Mechanism Leads to Shortened Chromosome Ends
Scientists at the University of Göttingen have deciphered the biogenesis of an enzyme complex whose role is to ensure that the ends of chromosomes are…
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Correlated Optical and Isotopic Nanoscopy
The isotopic composition of different materials can be imaged by secondary ion mass spectrometry. In biology, this method is mainly used to study…
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Single-Cell Phenotyping within Transparent Intact Tissue through Whole-Body Clearing
Understanding the structure-function relationships at cellular, circuit, and organ-wide scale requires 3D anatomical and phenotypical maps, currently…
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STED Microscopy of Living Cells – New Frontiers in Membrane and Neurobiology
Recent developments in fluorescence far-field microscopy such as STED microscopy have accomplished observation of the living cell with a spatial…
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Glucose-Regulated Protein 78 (Grp78) Confers Chemoresistance to Tumor Endothelial Cells under Acidic Stress
This study was designed to investigate the activation of the unfolded protein response (UPR) in tumor associated endothelial cells (TECs) and its…
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Super-resolution Molecular and Functional Imaging of Nanoscale Architectures in Life and Materials Science
Super-resolution (SR) fluorescence microscopy has been revolutionizing the way in which we investigate the structures, dynamics, and functions of a…
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Imaging Pheromone Sensing in a Mouse Vomeronasal Acute Tissue Slice Preparation
In mice, the ability to detect pheromones is principally mediated by the vomeronasal organ (VNO). Here, an acute tissue slice preparation of VNO for…