Science Lab

Science Lab

The knowledge portal of Leica Microsystems offers scientific research and teaching material on the subjects of microscopy. The content is designed to support beginners, experienced practitioners and scientists alike in their everyday work and experiments. Explore interactive tutorials and application notes, discover the basics of microscopy as well as high-end technologies – become part of the Science Lab community and share your expertise!

Latest Articles

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Correlated Optical and Isotopic Nanoscopy
The isotopic composition of different materials can be imaged by secondary ion mass spectrometry. In biology, this method is mainly used to study…
Aug 22, 2014
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Single-Cell Phenotyping within Transparent Intact Tissue through Whole-Body Clearing
Understanding the structure-function relationships at cellular, circuit, and organ-wide scale requires 3D anatomical and phenotypical maps, currently…
Aug 20, 2014
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STED Microscopy of Living Cells – New Frontiers in Membrane and Neurobiology
Recent developments in fluorescence far-field microscopy such as STED microscopy have accomplished observation of the living cell with a spatial…
Aug 12, 2014
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Glucose-Regulated Protein 78 (Grp78) Confers Chemoresistance to Tumor Endothelial Cells under Acidic Stress
This study was designed to investigate the activation of the unfolded protein response (UPR) in tumor associated endothelial cells (TECs) and its…
Jul 21, 2014
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Super-resolution Molecular and Functional Imaging of Nanoscale Architectures in Life and Materials Science
Super-resolution (SR) fluorescence microscopy has been revolutionizing the way in which we investigate the structures, dynamics, and functions of a…
Jul 07, 2014
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Imaging Pheromone Sensing in a Mouse Vomeronasal Acute Tissue Slice Preparation
In mice, the ability to detect pheromones is principally mediated by the vomeronasal organ (VNO). Here, an acute tissue slice preparation of VNO for…
May 02, 2014
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An Innovative Non-contact Method to Determine Surface Free Energy on Micro-areas
Surface free energy (SFE) is a property which depends on the chemical state and roughness of the surface and it is necessary to develop a reliable…
Apr 28, 2014
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Focus on Microscopy 2014 – Abstracts of the Presentations Online Available
Focus on Microscopy 2014 was held at the University of Sydney, Australia from April 13–16. Over 600 delegates and exhibitors attend this year’s FOM…
Apr 25, 2014
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Surface Roughness of Flowable Resin Composites eroded by Acidic and Alcoholic Drinks
Acidic beverages, such as soft drinks (orange juice, and cola), or ethanol (whisky), can produce erosion of resin composites. The surface degradation…
Apr 24, 2014
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Visualization of the Immunological Synapse by Dual Color Time-gated Stimulated Emission Depletion (STED) Nanoscopy
Here we illustrate the protocol for imaging by two-color STED nanoscopy the cytotoxic immune synapse of NK cells recapitulated on glass. Using this…
Mar 28, 2014
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A New Filtering Technique for Removing Anti-Stokes Emission Background in Gated CW-STED Microscopy
Stimulated emission depletion (STED) microscopy is a prominent approach of super-resolution optical microscopy, which allows cellular imaging with so…
Mar 25, 2014
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Genomic Survey, Gene Expression Analysis and Structural Modeling Suggest Diverse Roles of DNA Methyltransferases in Legumes
DNA methylation plays a crucial role in development through inheritable gene silencing. Plants possess three types of DNA methyltransferases (MTases),…
Mar 14, 2014
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Glucose-Stimulated Calcium Dynamics in Islets of Langerhans in Acute Mouse Pancreas Tissue Slices
In endocrine cells within islets of Langerhans calcium ions couple cell stimulation to hormone secretion. Since the advent of modern fluorimetry,…
Mar 11, 2014
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Production of RNA for Transcriptomic Analysis from Mouse Spinal Cord Motor Neuron Cell Bodies by Laser Capture Microdissection
High-quality total RNA has been prepared from cell bodies of mouse spinal cord motor neurons by laser capture microdissection after staining spinal…
Jan 30, 2014
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Novel Fluorescent Carbonic Nanomaterials for Sensing and Imaging
Small brightly fluorescent carbon nanoparticles have emerged as a new class of materials important for sensing and imaging applications. We analyze…
Jan 16, 2014
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The Relationship Between Membrane Potential and Calcium Dynamics in Glucose-Stimulated Beta Cell Syncytium in Acute Mouse Pancreas Tissue Slices
Oscillatory electrical activity is regarded as a hallmark of the pancreatic beta cell glucose-dependent excitability pattern. Electrophysiologically…
Dec 17, 2013
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Patch Clamp Recordings from Embryonic Zebrafish Mauthner Cells
Mauthner cells (M-cells) are large reticulospinal neurons located in the hindbrain of teleost fish. They are key neurons involved in a characteristic…
Oct 11, 2013
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STED Microscopy: A New Chapter in Light Imaging
When Stefan Hell invented a technique that pushed beyond the diffraction limit of light for the first time, a new field of nanoscale imaging was…
Sep 16, 2013
Abstract

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