Learn & Share: TIRF Microscopy

Total internal reflection fluorescence microscopy (TIRFM) is a highly sensitive technique to perform functional investigations in living cells. The high signal to noise ratio and a resolution in z direction of usually 70-250 nm above the coverslip/water interface allows to visualize and to analyse vesicles transport and signalling events, as well as kinetic studies and single molecules detection. Physical background is the total reflection of a laser beam at the interface of glass and water and the resulting electromagnetic wave, the so-called evanescent field. The energy of the evanescent field decreases exponentially with the distance of the interface coverslip/water and allows exciting fluorochromes.

Useful Links

Communities and Web Sources

www.researchgate.net/Social network for scientists

www.ibiology.org/Teaching tools, video lectures on biology and microscopy

bitesizebio.comOnline magazine and community for molecular and cell biology researchers

www.somersault1824.comResource for high-end scientific illustrations, images and animations

Search Engines and Data Bases

www.cellimagelibrary.orgPublic resource database of images, videos, and animations of cells

harvester.fzk.de/harvesterBioinformatic meta search engine for genes and proteins

www.gopubmed.comSearch interface for pubmed

en.wikipedia.org/wiki/List_of_academic_databases_and_search_enginesList of academic databases and search engines

scholar.google.comBeta of Google's search engine for scientific article abstracts


www.doaj.org/Directory of open access journals

emboj.embopress.org/The EMBO Journal

www.lifescied.orgCBE-Life Sciences Education – an ASCB online journal



www.cell.com/Biweekly publication of exceptional research articles

jcs.biologists.org/Journal of Cell Science


jeb.biologists.org/The Journal of Experimental Biology

dmm.biologists.org/DMM Disease Models & Mechanisms

www.biotechniques.com/International Journal of Life Science Methods

www.opticsinfobase.org/Collection of Journals and Proceedings in Optics and Photonics

spie.org/x576.xmlSPIE - peer-reviewed journals on applied research in optics and photonics

onlinelibrary.wiley.com/journal/10.1002/(ISSN)1864-0648Journal of Biophotonics

www.plosone.org/home.actionInternational, peer-reviewed, open-access, online publication

rspb.royalsocietypublishing.org/Proceedings B - the Royal Society's biological research journal

www.microscopy-analysis.com/International Journal for microscopists

Organizations / Institutes

www.microscopy.org/Microscopy Society of America

www.eurmicsoc.org/European Microscopy Society

www.rms.org.uk/Royal Microscopical Society

http://www.doitpoms.ac.ukTutorials on microscopy for materials science; University of Cambridge, Department of Materials Science and Metallurgy

www.ascb.org/ASCB American Society of Cell Biology

www.biologists.com/cob_activities.htmlthe company of biologists

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