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Learn & Share: Multiphoton Microscopy

With multiphoton microscopy we can image several hundreds of micrometers deep into biological samples as scattering of red-shifted light in tissues is highly reduced compared to visible light.  For multiphoton excitation, pulsed infrared lasers with wavelengths of up to 1300 nm in case of the OPO (optical-parametric oscillator, What is an OPO?) are used.

Deep tissue imaging of thick specimens or in whole animals allows to observe cellular processes in their natural context. Especially intravital imaging with multiphoton excitation plays a growing role in many biomedical research areas.

Useful Links

Communities and Web Sources network for scientists Microscopy Mailing List, University of Minnesota tools, video lectures on biology and microscopy

bitesizebio.comOnline magazine and community for molecular and cell biology researchers

www.somersault1824.comResource for high-end scientific illustrations, images and animations

Search Engines and Data Bases

www.cellimagelibrary.orgPublic resource database of images, videos, and animations of cells meta search engine for genes and proteins

www.gopubmed.comSearch interface for pubmed of academic databases and search engines of Google's search engine for scientific article abstracts

Journals of open access journals EMBO Journal

www.lifescied.orgCBE-Life Sciences Education – an ASCB online journal publication of exceptional research articles of Cell Science Journal of Experimental Biology Disease Models & Mechanisms Journal of Life Science Methods of Journals and Proceedings in Optics and Photonics - peer-reviewed journals on applied research in optics and photonics of Biophotonics, peer-reviewed, open-access, online publication B - the Royal Society's biological research journal Journal for microscopists

Organizations Society of America Microscopy Society Microscopical Society American Society of Cell Biology company of biologists

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