Science Lab

Science Lab


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Widefield Calcium Imaging with Calcium Indicator Fura2
In eukaryotic cells Ca2+ is one of the most widespread second messengers used in signal transduction pathways. Intracellular levels of Ca2+ are…
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Brief Introduction to Critical Point Drying
One of the uses of the Scanning Electron Microscope (SEM) is in the study of surface morphology in biological applications which requires the…
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3D Visualization of Surface Structures
One of the main features of a digital microscope is the speed and ease with which it enables surface models to be created of macroscopic and…
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Video Tutorial: How to Align the Bulb of a Fluorescence Lamp Housing
The traditional light source for fluorescence excitation is a fluorescence lamp housing with mercury burner. A prerequisite for achieving bright and…
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Video Tutorial: How to Change the Bulb of a Fluorescence Lamp Housing
When applying fluorescence microscopy in biological applications, a lamp housing with mercury burner is the most common light source. This video…
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FRET with FLIM
FLIM combines lifetime measurements with imaging: lifetimes obtained for each image pixel are color-coded to produce additional image contrast. Thus,…
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Fluorescence Correlation Spectroscopy
Fluorescence correlation spectroscopy (FCS) measures fluctuations of fluorescence intensity in a sub-femtolitre volume to detect such parameters as…
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Widefield Super-Resolution with GSDIM
Great advancements in biology have been possible by using fluorescence microscopy. So far, the resolution of the images was limited due to physical…
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How to Clean Microscope Optics
Clean microscope optics are essential for obtaining good microscope images. If they are dirty, the microscope should be cleaned to avoid a loss of…
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Image Processing for Widefield Microscopy
Fluorescence microscopy is a modern and steadily evolving tool to bring light to current cell biological questions. With the help of fluorescent…
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White Light Laser
The perfect light source for confocal microscopes in biomedical applications has sufficient intensity, tunable color and is pulsed for use in lifetime…
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Optical Microscopes – Some Basics
The optical microscope has been a standard tool in life science as well as material science for more than one and a half centuries now. To use this…
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Controlling the TIRF Penetration Depth is Mandatory for Reproducible Results
The main feature of total internal reflection fluorescence (TIRF) microscopy is the employment of an evanescent wave for the excitation of…
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Quantitative Fluorescence
Seeing is believing – and measuring is knowing. Microscopes generate images that are not only used for illustration, but are also subject to…
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Basic Principles of Luminescence
There are a lot of light-emitting processes occurring in nature. Luminescence is an umbrella term for those kinds of events where light emission is…
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Fluorescent Proteins – Introduction and Photo Spectral Characteristics
The prospects of fluorescence microscopy changed dramatically with the discovery of fluorescent proteins in the 1950s. The starting point was the…
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Introduction to Live-Cell Imaging
The understanding of complex and fast cellular dynamics is an important step to get insight into biological processes. Therefore, today’s life science…
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Total Internal Reflection Fluorescence (TIRF) Microscopy
Total internal reflection fluorescence (TIRF) is a special technique in fluorescence microscopy developed by Daniel Axelrod at the University of…

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