The Microscopy Knowledge Portal

Metabolic imaging based on fluorescence lifetime provides insights into the metabolic dynamics of cells, but its use has been limited as expertise in…

During the last decade, 3D cell culture has been established as a more realistic model compared to classical 2D culture systems. Cells can develop…

With the Leica Nano Workflow, searching for a needle in the haystack is a thing of the past. Take advantage of correlative light and electron…

Life is fast, especially for a cell. As a rule, cells should be examined under physiological conditions which are as close as possible to their…

3D cell cultures, such as organoids and spheroids, give insights into cells and their interactions with their microenvironment. These 3D cell cultures…

Phasor analysis for fluorescence lifetime imaging microscopy (FLIM) is a powerful and fitting-free tool that provides a 2D graphical assessment of…

In this article, a ratiometric fluorometric method for cytosolic calcium ion (Ca2+) measurement in cultured melanoma cells using Fura 2-AM cell…

We demonstrate residual channel attention networks for restoring and enhancing volumetric time-lapse (4D) fluorescence microscopy data.

Phenotype-genotype correlations are key for insight. From Eye to Insight is therefore fitting perfectly to Leica Microsystems and in particular to…

In life science research, live cell imaging is an indispensable tool to visualize cells in a state as in vivo as possible. This E-book reviews a wide…

The zebrafish larval brain is small and contains about 1 million times less neurons than the human counterpart. The neural circuitry and the general…

Leica Microsystems is a pioneer in the field of fluorescence microscopy technology, developing innovative fluorescent modules for various medical…

This publication is a compilation of appropriate workflows for the most frequently used sample preparation methods, like Correlative Methodologies,…

The SARS-CoV-2 outbreak started in late December 2019 and has since reached a global pandemic, leading to a worldwide battle against COVID-19. The…

Today, ion beam milling is one of the most widely-used methods for preparing samples for electron microscopy. Download this 76-pages booklet today and…

Dr. Mace will describe new advances in our understanding of the regulation of human NK cell actin cytoskeletal remodeling in cell migration and immune…

Molecular imaging of living specimens offers a means to draw upon the growing body of high-throughput molecular data to better understand the…

We have developed a microscopy-based pipeline to characterize a developmentally critical behavior at the pupal stage of development, called the…