Exploring Morphological and Biochemical Linkages in Fungal Growth with Label-Free Light Sheet Microscopy and Raman Spectroscopy.Siddhanta, S., 2016
Learn & Share: Light Sheet Microscopy
Living specimens, particularly developing embryos, are often sensitive to light exposure. Light sheet microscopy – also referred to as single plane illumination microscopy or SPIM – is a gentle way of imaging sensitive samples or fast biological processes in vivo. The specimen is illuminated only in a single plane at a time and detected from the perpendicular direction. Since there is no out-of-focus excitation, phototoxic effects are reduced to the focal plane. Light sheet imaging has intrinsic optical sectioning. By moving the sample through the light sheet, 3D images of a specimen can be recorded.
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Effects of Clearing Media on Tissue Transparency and Shrinkage
This study comprehensively evaluates the effects of different clearing media on tissue transparency and shrinkage by comparing freshly dissected dipteran fly brains with their cleared equivalents.…Read article
Following Ca²⁺ Changes in Acutely Stimulated Spheroids
Spheroids are easy and cost-effective 3D cell cultures, useful for fundamental and applied research. They can bridge the gap between 2D and in vivo experiments and can be used for high-throughput…Read article
Analyzing Structural and Functional Testicular Anatomy
Fleck, Kenzler et al. have shown that, in mice, muscle-like cells are located within the walls of seminiferous tubules. Using CLARITY and a Leica digital light-sheet (DLS) microscope, Fleck, Kenzler…Read article
Fish and Chips Sheet into the Light
The zebrafish larval brain is small and contains about 1 million times less neurons than the human counterpart. The neural circuitry and the general bauplan of the vertebrate brain, however, is…Read article
Understanding Motor Sequence Generation Across Spatiotemporal Scales
We have developed a microscopy-based pipeline to characterize a developmentally critical behavior at the pupal stage of development, called the ecdysis sequence. We study brain-wide neuronal activity…Read article
A Quality Metric for the Systematic Evaluation of Clearing Protocols
3D multicellular spheroids are of interest for studying tumor behavior and evaluating the response of pharmacologically active agents, because they mimic the in vivo tumor environment better than…Read article
Using Mounting Frames for DLS Sample Preparation
Sample handling is an important topic in the context of Light Sheet Microscopy. The TCS SP8 DLS integrates Light Sheet technology into an inverted confocal platform and can hence make use of general…Read article
Using a Rotation Device for DLS Sample Mounting
The TCS SP8 DLS from Leica Microsystems is an innovative concept to integrate the Light Sheet Microscopy technology into the confocal microscope. Due to its unique optical architecture samples can be…Read article
DLS Sample Preparation: Using U-Shaped Glass Capillaries for Sample Mounting
The TCS SP8 DLS microscope system from Leica Microsystems is an innovative concept which integrates the Light Sheet Microscopy technology into the SP8 confocal platform. Due to its unique optical…Read article
Clearing of Fixed Tissue: A Review from a Microscopist’s Perspective
Chemical clearing of fixed tissues is becoming a key instrument for the three-dimensional reconstruction of macroscopic tissue portions, including entire organs. Indeed, the growing interest in this…Read article
Super-Resolution Mapping of Neuronal Circuitry With an Index-Optimized Clearing Agent
Super-resolution imaging deep inside tissues has been challenging, as it is extremely sensitive to light scattering and spherical aberrations. Here, we report an optimized optical clearing agent for…Read article
Light Sheet Microscopy Turned Vertically
Living cells and organisms often suffer from the high light intensities used for fluorescent imaging. Light sheet microscopy reduces phototoxic effects and bleaching by illuminating a specimen in only…Read article
"The Leica Digital Light Sheet Module – a Clever Example of Thinking Out of the Box"
Bram van den Broek is a postdoctoral fellow at the Netherlands cancer institute in Amsterdam where he supports the advanced microscopy techniques in the laboratory of Kees Jalink. Working with Leica…Read article
Confocal and Digital Light Sheet Imaging
Optical imaging instrumentation can magnify tiny objects, zoom in on distant stars and reveal details that are invisible to the naked eye. But it notoriously suffers from an annoying problem: the…Read article
Nature Methods: Light-sheet Fluorescence Microscopy - Method of the Year 2014
Just about everyone who has examined fluorescent samples under the microscope is aware of the constant struggle to have enough signal to see the labeled structures while also avoiding fluorophore…Read article
Light Sheet Fluorescence Microscopy: Beyond the Flatlands
Light Sheet Fluorescence Microscopy (LISH-M) is a true fluorescence optical sectioning technique, first described by Heinrich Siedentopf in 1902 under the name of Ultramicroscopy. Light sheet…Read article
Quantitative Imaging in Cell Biology: Light Sheet Microscopy
This chapter introduces the concept of light sheet microscopy along with practical advice on how to design and build such an instrument. Selective plane illumination microscopy is presented as an…Read article
Light Sheet Fluorescence Microscopy - A Review
Light sheet fluorescence microscopy (LSFM) functions as a non-destructive microtome and microscope that uses a plane of light to optically section and view tissues with subcellular resolution. This…Read article
Useful Links
Publications on Light Sheet Microscopy
Visualization and targeting of LGR5+ human colon cancer stem cellsShimokawa, M. et al., 2017
Super-Resolution Mapping of Neuronal Circuitry With an Index-Optimized Clearing AgentKe, M.-T. et al., 2016
Light Sheet Microscopy to Measure Protein Dynamics.Rieckher, M., 2016
Further reading on Light Sheet Microscopy
www.sciencemag.org/content/305/5686/1007.longHuisken, J. et al., 2004
www.ncbi.nlm.nih.gov/pubmed/8371260?dopt=Abstract&holding=npgVoie, A. H. et al., 1993
Light Sheet Fluorescence Microscopy International Conference
www.lsfm2017.org/Singapore 2017
www.lsfm2016.org/Sheffield 2016
www.lsfm2015.org/Genoa 2015
www.lsfm2014.com/Barcelona 2014
Communities and Web Sources
www.researchgate.net/Social network for scientists
www.ibiology.org/Teaching tools, video lectures on biology and microscopy
www.bitesizebio.comOnline magazine and community for molecular and cell biology researchers
www.somersault1824.comResource for high-end scientific illustrations, images and animations
Search Engines and Data Bases
www.cellimagelibrary.orgPublic resource database of images, videos, and animations of cells
harvester.fzk.de/harvesterBioinformatic meta search engine for genes and proteins
www.gopubmed.comSearch interface for pubmed
en.wikipedia.org/wiki/List_of_academic_databases_and_search_enginesList of academic databases and search engines
scholar.google.comBeta of Google's search engine for scientific article abstracts
Journals
www.doaj.org/Directory of open access journals
emboj.embopress.org/The EMBO Journal
www.lifescied.orgCBE-Life Sciences Education – an ASCB online journal
www.sciencemag.org/Science
www.nature.com/Nature
www.cell.com/Biweekly publication of exceptional research articles
jcs.biologists.org/Journal of Cell Science
dev.biologists.org/Development
jeb.biologists.org/The Journal of Experimental Biology
dmm.biologists.org/DMM Disease Models & Mechanisms
www.biotechniques.com/
International Journal of Life Science Methods
www.opticsinfobase.org/Collection of Journals and Proceedings in Optics and Photonics
spie.org/x576.xmlSPIE - peer-reviewed journals on applied research in optics and photonics
onlinelibrary.wiley.com/journal/10.1002/(ISSN)1864-0648Journal of Biophotonics
www.plosone.org/home.actionInternational, peer-reviewed, open-access, online publication
rspb.royalsocietypublishing.org/Proceedings B - the Royal Society's biological research journal
www.microscopy-analysis.com/International Journal for microscopists
Organizations / Institutes
www.microscopy.org/Microscopy Society of America
www.eurmicsoc.org/European Microscopy Society
www.rms.org.uk/Royal Microscopical Society
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