Learn & Share: Light Sheet Microscopy

Living specimens, particularly developing embryos, are often sensitive to light exposure. Light sheet microscopy – also referred to as single plane illumination microscopy or SPIM – is a gentle way of imaging sensitive samples or fast biological processes in vivo. The specimen is illuminated only in a single plane at a time and detected from the perpendicular direction. Since there is no out-of-focus excitation, phototoxic effects are reduced to the focal plane. Light sheet imaging has intrinsic optical sectioning. By moving the sample through the light sheet, 3D images of a specimen can be recorded.

Useful Links

Publications on Light Sheet Microscopy

Visualization and targeting of LGR5+ human colon cancer stem cellsShimokawa, M. et al., 2017

Super-Resolution Mapping of Neuronal Circuitry With an Index-Optimized Clearing AgentKe, M.-T. et al., 2016

Light Sheet Microscopy to Measure Protein Dynamics.Rieckher, M., 2016

Further reading on Light Sheet Microscopy

www.sciencemag.org/content/305/5686/1007.longHuisken, J. et al., 2004

www.ncbi.nlm.nih.gov/pubmed/8371260?dopt=Abstract&holding=npgVoie, A. H. et al., 1993

Light Sheet Fluorescence Microscopy International Conference

www.lsfm2017.org/Singapore 2017

www.lsfm2016.org/Sheffield 2016

www.lsfm2015.org/Genoa 2015

www.lsfm2014.com/Barcelona 2014

Communities and Web Sources

www.researchgate.net/Social network for scientists

www.ibiology.org/Teaching tools, video lectures on biology and microscopy

www.bitesizebio.comOnline magazine and community for molecular and cell biology researchers

www.somersault1824.comResource for high-end scientific illustrations, images and animations

Search Engines and Data Bases

www.cellimagelibrary.orgPublic resource database of images, videos, and animations of cells

harvester.fzk.de/harvesterBioinformatic meta search engine for genes and proteins

www.gopubmed.comSearch interface for pubmed

en.wikipedia.org/wiki/List_of_academic_databases_and_search_enginesList of academic databases and search engines

scholar.google.comBeta of Google's search engine for scientific article abstracts


www.doaj.org/Directory of open access journals

emboj.embopress.org/The EMBO Journal

www.lifescied.orgCBE-Life Sciences Education – an ASCB online journal



www.cell.com/Biweekly publication of exceptional research articles

jcs.biologists.org/Journal of Cell Science


jeb.biologists.org/The Journal of Experimental Biology

dmm.biologists.org/DMM Disease Models & Mechanisms

International Journal of Life Science Methods

www.opticsinfobase.org/Collection of Journals and Proceedings in Optics and Photonics

spie.org/x576.xmlSPIE - peer-reviewed journals on applied research in optics and photonics

onlinelibrary.wiley.com/journal/10.1002/(ISSN)1864-0648Journal of Biophotonics

www.plosone.org/home.actionInternational, peer-reviewed, open-access, online publication

rspb.royalsocietypublishing.org/Proceedings B - the Royal Society's biological research journal

www.microscopy-analysis.com/International Journal for microscopists

Organizations / Institutes

www.microscopy.org/Microscopy Society of America

www.eurmicsoc.org/European Microscopy Society

www.rms.org.uk/Royal Microscopical Society

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