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Learn & Share: Correlative Light Electron Microscopy (CLEM)

CLEM (Correlative Light Electron Microscopy) combines the capabilities of two typically separate microscopy platforms: light (or fluorescent) microscopy (LM) and electron microscopy (EM). The advantage of LM is that it can provide wide field images of whole, often living, cells, but its resolution is limited. The advantage of EM is that it can provide much higher resolution images, up to molecular dimensions, but only over specific regions of a cell at a time and not in living cells. CLEM combines the advantages of both techniques, allowing scientists to spot cellular structures and processes of interest in whole cell images with LM and then zoom in for a closer look with EM. This dual examination provides valuable complementary and often unique information.

Useful Links

Communities network for scientists

bitesizebio.comOnline magazine and community for molecular and cell biology researchers

Search Engines and Data Bases

www.cellimagelibrary.orgPublic resource database of images, videos, and animations of cells meta search engine for genes and proteins

www.gopubmed.comSearch interface for pubmed of academic databases and search engines of Google's search engine for scientific article abstracts

www.somersault1824.comResource for high-end scientific illustrations, images and animations

Web Sources in Cell Biology – Vol. 111: Correlative Light and Electron Microscopy (Mueller-Reichert T, Verkade P) Biomedical Imaging: Principles and Applications (Salzer R), Chapter 6.5: CLEM Applications Leiden University: Correlative Light and Electron Microscopy: Strategies and Applications TeleThon Electron Microscopy Core Facility (TeEMCoF): Correlative Light-Electron Microscopy Correlative Light and Electron Microscopy Correlative Microscopy in Focus: Zoom-in Beyond LM Correlated Fluorescence and 3D Electron Microscopy with High Sensitivity and Spatial Precision Cell Microscopy Center: Techniques Methods: Correlative Light Electron Microscopy Correlative Microscopy Advances in High-resolution Imaging – Techniques for Three-dimensional Imaging of Cellular Structures A Single Probe to Combine 2 Imaging Modalities CLEM on Ultrathin Sections – From Micro- to Nanoscale Correlative Light and Electron Microscopic Exploration of Endocytic Organelles In and Out of Mitosis – Correlative Light and Electron Microscopy for Structural Cell Biology Cryo-fluorescence Microscopy Facilitates Correlations between Light and Cryo-electron Microscopy and Reduces the Rate of Photobleaching Correlative Light and Electron Microscopy: Visualization of Molecular Machines in Their Native Cell and Tissue Context The Use of High-pressure Freezing in Correlative Light Electron Microscopy Photooxidation Technology for Correlative Light and Electron Microscopy Multi-color Correlative Light and Electron Microscopy Using Nanoparticle Cathodoluminescence

Organizations Society of America Microscopy Society Microscopical Society American Society of Cell Biology company of biologists

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