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Correlative Light Electron Microscopy (CLEM)

CLEM (Correlative Light Electron Microscopy) combines the capabilities of two typically separate microscopy platforms: light (or fluorescent) microscopy (LM) and electron microscopy (EM). The advantage of LM is that it can provide wide field images of whole, often living, cells, but its resolution is limited. The advantage of EM is that it can provide much higher resolution images, up to molecular dimensions, but only over specific regions of a cell at a time and not in living cells. CLEM combines the advantages of both techniques, allowing scientists to spot cellular structures and processes of interest in whole cell images with LM and then zoom in for a closer look with EM. This dual examination provides valuable complementary and often unique information.



Useful Links

Social network for scientists
Online magazine and community for molecular and cell biology researchers

Search Engines and Data Bases
Public resource database of images, videos, and animations of cells
Bioinformatic meta search engine for genes and proteins
Search interface for pubmed
List of academic databases and search engines
Beta of Google's search engine for scientific article abstracts
Resource for high-end scientific illustrations, images and animations

Web Sources
Methods in Cell Biology – Vol. 111: Correlative Light and Electron Microscopy (Mueller-Reichert T, Verkade P)
Biomedical Imaging: Principles and Applications (Salzer R), Chapter 6.5: CLEM Applications
Leiden University: Correlative Light and Electron Microscopy: Strategies and Applications
The TeleThon Electron Microscopy Core Facility (TeEMCoF): Correlative Light-Electron Microscopy
Volume Correlative Light and Electron Microscopy
Correlative Microscopy in Focus: Zoom-in Beyond LM
Correlated Fluorescence and 3D Electron Microscopy with High Sensitivity and Spatial Precision
Cell Microscopy Center: Techniques
Methods: Correlative Light Electron Microscopy
Correlative Microscopy
Advances in High-resolution Imaging – Techniques for Three-dimensional Imaging of Cellular Structures
A Single Probe to Combine 2 Imaging Modalities
CLEM on Ultrathin Sections – From Micro- to Nanoscale
Correlative Light and Electron Microscopic Exploration of Endocytic Organelles
In and Out of Mitosis – Correlative Light and Electron Microscopy for Structural Cell Biology
Cryo-fluorescence Microscopy Facilitates Correlations between Light and Cryo-electron Microscopy and Reduces the Rate of Photobleaching
Correlative Light and Electron Microscopy: Visualization of Molecular Machines in Their Native Cell and Tissue Context
The Use of High-pressure Freezing in Correlative Light Electron Microscopy
Photooxidation Technology for Correlative Light and Electron Microscopy
Multi-color Correlative Light and Electron Microscopy Using Nanoparticle Cathodoluminescence

Microscopy Society of America
European Microscopy Society
Royal Microscopical Society
ASCB American Society of Cell Biology
the company of biologists

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