Learn & Share: Correlative Light Electron Microscopy (CLEM)
CLEM (Correlative Light Electron Microscopy) combines the capabilities of two typically separate microscopy platforms: light (or fluorescent) microscopy (LM) and electron microscopy (EM). The advantage of LM is that it can provide wide field images of whole, often living, cells, but its resolution is limited. The advantage of EM is that it can provide much higher resolution images, up to molecular dimensions, but only over specific regions of a cell at a time and not in living cells. CLEM combines the advantages of both techniques, allowing scientists to spot cellular structures and processes of interest in whole cell images with LM and then zoom in for a closer look with EM. This dual examination provides valuable complementary and often unique information.
Targeting Active Recycling Nuclear Pore Complexes using Cryo Confocal Microscopy
In this article, how cryo light microscopy and, in particular cryo confocal microscopy, is used to improve the reliability of cryo EM workflows is described. The quality of the EM grids and samples is…Read article
Advancing Cell Biology with Cryo-Correlative Microscopy
Correlative light and electron microscopy (CLEM) advances biological discoveries by merging different microscopes and imaging modalities to study systems in 4D. Combining fluorescence microscopy with…Read article
Crystal clear cryo light-microscopy images
This article describes how computational clearing of cryo light microscopy images improves the identification of cellular targets for cryo electron-microscopy.Read article
Microscopy in Virology
The coronavirus SARS-CoV-2, causing the Covid-19 disease effects our world in all aspects. Research to find immunization and treatment methods, in other words to fight this virus, gained highest…Read article
Introduction to Ion Beam Etching with the EM TIC 3X
In this article you can learn how to optimize the preparation quality of your samples by using the ion beam etching method with the EM TIC 3X ion beam milling machine. A short introduction of the…Read article
Improve Cryo Electron Tomography Workflow
Leica Microsystems and Thermo Fisher Scientific have collaborated to create a fully integrated cryo-tomography workflow that responds to these research needs: Reveal cellular mechanisms at…Read article
Correlative Cryo-Fluorescence and Cryo-Scanning Electron Microscopy as a Straightforward Tool to Study Host-Pathogen Interactions
Correlative light and electron microscopy is an imaging technique that enables identification and targeting of fluorescently tagged structures with subsequent imaging at near-to-nanometer resolution.…Read article
Imaging of Host Cell-bacteria Interactions using Correlative Microscopy under Cryo-conditions
Pathogenic bacteria have developed intriguing strategies to establish and promote infections in their respective hosts. Most bacterial pathogens initiate infectious diseases by adhering to host cells…Read article
Correlative In-Resin Super-Resolution and Electron Microscopy Using Standard Fluorescent Proteins
We introduce a method for correlative in-resin super-resolution fluorescence and electron microscopy (EM) of biological structures in mammalian culture cells. Cryo-fixed resin embedded samples offer…Read article
Correlative Light and Electron Microscopy – Get Your Free CLEM e-Book for Download
The urge to go deeper into the microscopic world has led researchers to combine the versatility of the light microscopy (LM) with the resolution power of the electron microscope (EM) to produce…Read article
Correlating Intravital Multi-Photon Microscopy to 3D Electron Microscopy of Invading Tumor Cells Using Anatomical Reference Points
Cancer research unsing multiphoton microscopy and 3D electron microscopy. Correlative microscopy combines the advantages of both light and electron microscopy to enable imaging of rare and transient…Read article
Customized Patterned Substrates for Highly Versatile Correlative Light-Scanning Electron Microscopy
Correlative light electron microscopy (CLEM) combines the advantages of light and electron microscopy, thus making it possible to follow dynamic events in living cells at nanometre resolution. Various…Read article
New Insights into Cilia and Flagella by Cryo-EM
Cilia and flagella were the first organelles to be discovered and have been studied for centuries. However, their essential role in humans and how ciliary defects cause diseases are still not well…Read article
ICln: A New Regulator of Non-Erythroid 4.1R Localisation and Function
To optimise the efficiency of cell machinery, cells can use the same protein (often called a hub protein) to participate in different cell functions by simply changing its target molecules. There are…Read article
A Precise and Rapid Mapping Protocol for Correlative Light and Electron Microscopy of small invertebrate organisms
CLEM (correlative live cell and electronmicroscopy) seeks to bridge the data acquired with different imaging strategies, typically between light microscopy and electron microscopy. It has been…Read article
Brief Introduction to High-Pressure Freezing
Water is the most abundant cellular constituent and therefore important for preserving cellular ultra-structure. Currently the only way to fix cellular constituents without introducing significant…Read article
Postlipolytic Insulin-dependent Remodeling of Micro Lipid Droplets in Adipocytes
Despite the lipolysis–lipogenesis cycle being a fundamental process in adipocyte biology, very little is known about the morphological changes that occur during this process. The remodeling of lipid…Read article
A Genetically Encoded Tag for Correlated Light and Electron Microscopy of Intact Cells, Tissues, and Organisms
Electron microscopy (EM) achieves the highest spatial resolution in protein localization, but specific protein EM labeling has lacked generally applicable genetically encoded tags for in situ…Read article
Advanced Correlative Light/Electron Microscopy: Current Methods and New Developments Using Tokuyasu Cryosections
Microscopy is an essential tool for analysis of cellular structures and function. With the advent of new fluorescent probes and super-resolution light microscopy techniques, the study of dynamic…Read article
High Data Output and Automated 3D Correlative Light–Electron Microscopy Method
Correlative light/electron microscopy (CLEM) allows the simultaneous observation of a given subcellular structure by fluorescence light microscopy (FLM) and electron microscopy. The use of this…Read article
CLEM: Combining the Strengths of Light and Electron Microscopy
In recent years light microscopy studies have been dominated by live cell imaging while electron microscopy has been used for high-resolution studies. Latterly, there has been increasing interest in…Read article
Useful Links
Communities
www.researchgate.net/Social network for scientists
bitesizebio.comOnline magazine and community for molecular and cell biology researchers
Search Engines and Data Bases
www.cellimagelibrary.orgPublic resource database of images, videos, and animations of cells
harvester.fzk.de/harvesterBioinformatic meta search engine for genes and proteins
www.gopubmed.comSearch interface for pubmed
en.wikipedia.org/wiki/List_of_academic_databases_and_search_enginesList of academic databases and search engines
scholar.google.comBeta of Google's search engine for scientific article abstracts
www.somersault1824.comResource for high-end scientific illustrations, images and animations
Web Sources
https://www.elsevier.com/books/correlative-light-and-electron-microscopy/unknown/978-0-12-416026-2Methods in Cell Biology – Vol. 111: Correlative Light and Electron Microscopy (Mueller-Reichert T, Verkade P)
http://my.safaribooksonline.com/book/-/9781118271926/chapter-6-toward-a-3d-view-of-cellular-architecture-correlative-light-microscopy/c06_level1_5_xhtml Biomedical Imaging: Principles and Applications (Salzer R), Chapter 6.5: CLEM Applications
https://openaccess.leidenuniv.nl/handle/1887/17922 Leiden University: Correlative Light and Electron Microscopy: Strategies and Applications
http://teemcof.ibp.cnr.it/services/correlative-light-electron-microscopyThe TeleThon Electron Microscopy Core Facility (TeEMCoF): Correlative Light-Electron Microscopy
www.embl.de/research/units/cbb/schwab/Volume Correlative Light and Electron Microscopy
www.emez.ethz.ch/education/pastevents/pastev/Correl_LMEM_rw.pdf Correlative Microscopy in Focus: Zoom-in Beyond LM …
http://jcb.rupress.org/content/192/1/111.full Correlated Fluorescence and 3D Electron Microscopy with High Sensitivity and Spatial Precision
www.cellbiology-utrecht.nl/second-menu-cmc/techniques.html Cell Microscopy Center: Techniques
www.biocenter.helsinki.fi/bi/em/emu_methods_clem.html Methods: Correlative Light Electron Microscopy
www.i-bioimaging.it/images/Alberto%20Luini.pdf Correlative Microscopy
http://jcs.biologists.org/content/early/2012/06/05/jcs.090027.full.pdf Advances in High-resolution Imaging – Techniques for Three-dimensional Imaging of Cellular Structures
www.sartre.ac.uk/sites/default/files/Verkade.pdf A Single Probe to Combine 2 Imaging Modalities
www.imaging-git.com/science/light-microscopy/clem-ultrathin-sections CLEM on Ultrathin Sections – From Micro- to Nanoscale
www.formatex.info/microscopy4/277-285.pdf Correlative Light and Electron Microscopic Exploration of Endocytic Organelles
www.emc2012.org.uk//documents/Abstracts/Abstracts/EMC2012_1163.pdf In and Out of Mitosis – Correlative Light and Electron Microscopy for Structural Cell Biology
http://users.unimi.it/~cima/bibliocorrelative/jmicroscopy2007.pdf Cryo-fluorescence Microscopy Facilitates Correlations between Light and Cryo-electron Microscopy and Reduces the Rate of Photobleaching
www.cgl.ucsf.edu/Research/auer/ Correlative Light and Electron Microscopy: Visualization of Molecular Machines in Their Native Cell and Tissue Context
www.labmate-online.com/articles/microscopy-and-microtechniques/4/paul_verkade/the_use_of_high-pressure_freezing_in_correlative_light_electron_microscopy/71/ The Use of High-pressure Freezing in Correlative Light Electron Microscopy
http://de.scribd.com/doc/156152708/Photooxidation-Technology-for-Correlative-Light-and-Electron-Microscopy Photooxidation Technology for Correlative Light and Electron Microscopy
http://walsworth.physics.harvard.edu/publications/2012_Glenn_ArXiv.pdf Multi-color Correlative Light and Electron Microscopy Using Nanoparticle Cathodoluminescence
Organizations
www.microscopy.org/Microscopy Society of America
www.eurmicsoc.org/European Microscopy Society
www.rms.org.uk/Royal Microscopical Society
www.ascb.org/ASCB American Society of Cell Biology
www.biologists.com/cob_activities.htmlthe company of biologists
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