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Correlative Light Electron Microscopy (CLEM)

CLEM (Correlative Light Electron Microscopy) combines the capabilities of two typically separate microscopy platforms: light (or fluorescent) microscopy (LM) and electron microscopy (EM). The advantage of LM is that it can provide wide field images of whole, often living, cells, but its resolution is limited. The advantage of EM is that it can provide much higher resolution images, up to molecular dimensions, but only over specific regions of a cell at a time and not in living cells. CLEM combines the advantages of both techniques, allowing scientists to spot cellular structures and processes of interest in whole cell images with LM and then zoom in for a closer look with EM. This dual examination provides valuable complementary and often unique information.

 

 

Useful Links

Communities

www.researchgate.net/
Social network for scientists

bitesizebio.com
Online magazine and community for molecular and cell biology researchers

Search Engines and Data Bases

www.cellimagelibrary.org
Public resource database of images, videos, and animations of cells

harvester.fzk.de/harvester
Bioinformatic meta search engine for genes and proteins

www.gopubmed.com
Search interface for pubmed

en.wikipedia.org/wiki/List_of_academic_databases_and_search_engines
List of academic databases and search engines

scholar.google.com
Beta of Google's search engine for scientific article abstracts

www.somersault1824.com
Resource for high-end scientific illustrations, images and animations

Web Sources

https://www.elsevier.com/books/correlative-light-and-electron-microscopy/unknown/978-0-12-416026-2
Methods in Cell Biology – Vol. 111: Correlative Light and Electron Microscopy (Mueller-Reichert T, Verkade P)

http://my.safaribooksonline.com/book/-/9781118271926/chapter-6-toward-a-3d-view-of-cellular-architecture-correlative-light-microscopy/c06_level1_5_xhtml
Biomedical Imaging: Principles and Applications (Salzer R), Chapter 6.5: CLEM Applications

https://openaccess.leidenuniv.nl/handle/1887/17922
Leiden University: Correlative Light and Electron Microscopy: Strategies and Applications

http://teemcof.ibp.cnr.it/services/correlative-light-electron-microscopy
The TeleThon Electron Microscopy Core Facility (TeEMCoF): Correlative Light-Electron Microscopy

www.embl.de/research/units/cbb/schwab/
Volume Correlative Light and Electron Microscopy

www.emez.ethz.ch/education/pastevents/pastev/Correl_LMEM_rw.pdf
Correlative Microscopy in Focus: Zoom-in Beyond LM

http://jcb.rupress.org/content/192/1/111.full
Correlated Fluorescence and 3D Electron Microscopy with High Sensitivity and Spatial Precision

www.cellbiology-utrecht.nl/second-menu-cmc/techniques.html
Cell Microscopy Center: Techniques

www.biocenter.helsinki.fi/bi/em/emu_methods_clem.html
Methods: Correlative Light Electron Microscopy

www.i-bioimaging.it/images/Alberto%20Luini.pdf
Correlative Microscopy

http://jcs.biologists.org/content/early/2012/06/05/jcs.090027.full.pdf
Advances in High-resolution Imaging – Techniques for Three-dimensional Imaging of Cellular Structures

www.sartre.ac.uk/sites/default/files/Verkade.pdf
A Single Probe to Combine 2 Imaging Modalities

www.imaging-git.com/science/light-microscopy/clem-ultrathin-sections
CLEM on Ultrathin Sections – From Micro- to Nanoscale

www.formatex.info/microscopy4/277-285.pdf
Correlative Light and Electron Microscopic Exploration of Endocytic Organelles

www.emc2012.org.uk//documents/Abstracts/Abstracts/EMC2012_1163.pdf
In and Out of Mitosis – Correlative Light and Electron Microscopy for Structural Cell Biology

http://users.unimi.it/~cima/bibliocorrelative/jmicroscopy2007.pdf
Cryo-fluorescence Microscopy Facilitates Correlations between Light and Cryo-electron Microscopy and Reduces the Rate of Photobleaching

www.cgl.ucsf.edu/Research/auer/
Correlative Light and Electron Microscopy: Visualization of Molecular Machines in Their Native Cell and Tissue Context

www.labmate-online.com/articles/microscopy-and-microtechniques/4/paul_verkade/the_use_of_high-pressure_freezing_in_correlative_light_electron_microscopy/71/
The Use of High-pressure Freezing in Correlative Light Electron Microscopy

http://de.scribd.com/doc/156152708/Photooxidation-Technology-for-Correlative-Light-and-Electron-Microscopy
Photooxidation Technology for Correlative Light and Electron Microscopy

http://walsworth.physics.harvard.edu/publications/2012_Glenn_ArXiv.pdf
Multi-color Correlative Light and Electron Microscopy Using Nanoparticle Cathodoluminescence

Organizations

www.microscopy.org/
Microscopy Society of America

www.eurmicsoc.org/
European Microscopy Society

www.rms.org.uk/
Royal Microscopical Society

www.ascb.org/
ASCB American Society of Cell Biology

www.biologists.com/cob_activities.html
the company of biologists

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