Learn & Share: Correlative Light Electron Microscopy (CLEM)

CLEM (Correlative Light Electron Microscopy) combines the capabilities of two typically separate microscopy platforms: light (or fluorescent) microscopy (LM) and electron microscopy (EM). The advantage of LM is that it can provide wide field images of whole, often living, cells, but its resolution is limited. The advantage of EM is that it can provide much higher resolution images, up to molecular dimensions, but only over specific regions of a cell at a time and not in living cells. CLEM combines the advantages of both techniques, allowing scientists to spot cellular structures and processes of interest in whole cell images with LM and then zoom in for a closer look with EM. This dual examination provides valuable complementary and often unique information.

Useful Links


www.researchgate.net/Social network for scientists

bitesizebio.comOnline magazine and community for molecular and cell biology researchers

Search Engines and Data Bases

www.cellimagelibrary.orgPublic resource database of images, videos, and animations of cells

harvester.fzk.de/harvesterBioinformatic meta search engine for genes and proteins

www.gopubmed.comSearch interface for pubmed

en.wikipedia.org/wiki/List_of_academic_databases_and_search_enginesList of academic databases and search engines

scholar.google.comBeta of Google's search engine for scientific article abstracts

www.somersault1824.comResource for high-end scientific illustrations, images and animations

Web Sources

https://www.elsevier.com/books/correlative-light-and-electron-microscopy/unknown/978-0-12-416026-2Methods in Cell Biology – Vol. 111: Correlative Light and Electron Microscopy (Mueller-Reichert T, Verkade P)

http://my.safaribooksonline.com/book/-/9781118271926/chapter-6-toward-a-3d-view-of-cellular-architecture-correlative-light-microscopy/c06_level1_5_xhtml Biomedical Imaging: Principles and Applications (Salzer R), Chapter 6.5: CLEM Applications

https://openaccess.leidenuniv.nl/handle/1887/17922 Leiden University: Correlative Light and Electron Microscopy: Strategies and Applications

http://teemcof.ibp.cnr.it/services/correlative-light-electron-microscopyThe TeleThon Electron Microscopy Core Facility (TeEMCoF): Correlative Light-Electron Microscopy

www.embl.de/research/units/cbb/schwab/Volume Correlative Light and Electron Microscopy

www.emez.ethz.ch/education/pastevents/pastev/Correl_LMEM_rw.pdf Correlative Microscopy in Focus: Zoom-in Beyond LM

http://jcb.rupress.org/content/192/1/111.full Correlated Fluorescence and 3D Electron Microscopy with High Sensitivity and Spatial Precision

www.cellbiology-utrecht.nl/second-menu-cmc/techniques.html Cell Microscopy Center: Techniques

www.biocenter.helsinki.fi/bi/em/emu_methods_clem.html Methods: Correlative Light Electron Microscopy

www.i-bioimaging.it/images/Alberto%20Luini.pdf Correlative Microscopy

http://jcs.biologists.org/content/early/2012/06/05/jcs.090027.full.pdf Advances in High-resolution Imaging – Techniques for Three-dimensional Imaging of Cellular Structures

www.sartre.ac.uk/sites/default/files/Verkade.pdf A Single Probe to Combine 2 Imaging Modalities

www.imaging-git.com/science/light-microscopy/clem-ultrathin-sections CLEM on Ultrathin Sections – From Micro- to Nanoscale

www.formatex.info/microscopy4/277-285.pdf Correlative Light and Electron Microscopic Exploration of Endocytic Organelles

www.emc2012.org.uk//documents/Abstracts/Abstracts/EMC2012_1163.pdf In and Out of Mitosis – Correlative Light and Electron Microscopy for Structural Cell Biology

http://users.unimi.it/~cima/bibliocorrelative/jmicroscopy2007.pdf Cryo-fluorescence Microscopy Facilitates Correlations between Light and Cryo-electron Microscopy and Reduces the Rate of Photobleaching

www.cgl.ucsf.edu/Research/auer/ Correlative Light and Electron Microscopy: Visualization of Molecular Machines in Their Native Cell and Tissue Context

www.labmate-online.com/articles/microscopy-and-microtechniques/4/paul_verkade/the_use_of_high-pressure_freezing_in_correlative_light_electron_microscopy/71/ The Use of High-pressure Freezing in Correlative Light Electron Microscopy

http://de.scribd.com/doc/156152708/Photooxidation-Technology-for-Correlative-Light-and-Electron-Microscopy Photooxidation Technology for Correlative Light and Electron Microscopy

http://walsworth.physics.harvard.edu/publications/2012_Glenn_ArXiv.pdf Multi-color Correlative Light and Electron Microscopy Using Nanoparticle Cathodoluminescence


www.microscopy.org/Microscopy Society of America

www.eurmicsoc.org/European Microscopy Society

www.rms.org.uk/Royal Microscopical Society

www.ascb.org/ASCB American Society of Cell Biology

www.biologists.com/cob_activities.htmlthe company of biologists

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