Learn & Share: Correlative Light Electron Microscopy (CLEM)
CLEM (Correlative Light Electron Microscopy) combines the capabilities of two typically separate microscopy platforms: light (or fluorescent) microscopy (LM) and electron microscopy (EM). The advantage of LM is that it can provide wide field images of whole, often living, cells, but its resolution is limited. The advantage of EM is that it can provide much higher resolution images, up to molecular dimensions, but only over specific regions of a cell at a time and not in living cells. CLEM combines the advantages of both techniques, allowing scientists to spot cellular structures and processes of interest in whole cell images with LM and then zoom in for a closer look with EM. This dual examination provides valuable complementary and often unique information.
How to Target Fluorescent Structures in 3D for Cryo-FIB Milling
This article describes the major steps of the cryo-electron tomography workflow including super-resolution cryo-confocal microscopy. We describe how subcellular structures can be precisely located in…Read article
Advancing Cellular Ultrastructure Research
Freeze-fracture and freeze-etching are useful tools for studying flexible membrane-associated structures such as tight junctions or the enteric glycocalyx. Freeze-fracture and etching are two…Read article
How to Improve Live Cell Imaging with Coral Nano
For live-cell CLEM applications, light microscopy imaging is a critical step for identifying the right cell in the right state at the right time. In this article, Leica experts share their insights on…Read article
The Cryo-CLEM Journey
This article describes the Cryo-CLEM technology and the benefits it can provide for scientists. Additionally, some scientific publications are highlighted. Recent developments in cryo electron…Read article
Leica Nano Workflow Webinar
With the Leica Nano Workflow, searching for the needle in the haystack is a thing of the past. Take advantage of correlative light and electron microscopy to identify directly the right cell at the…Read article
Putting Dynamic Live Cell Data into the Ultrastructural Context
With the Leica Nano Workflow, searching for a needle in the haystack is a thing of the past. Take advantage of correlative light and electron microscopy to identify directly the right cell at the…Read article
Targeting Active Recycling Nuclear Pore Complexes using Cryo Confocal Microscopy
In this article, how cryo light microscopy and, in particular cryo confocal microscopy, is used to improve the reliability of cryo EM workflows is described. The quality of the EM grids and samples is…Read article
Advancing Cell Biology with Cryo-Correlative Microscopy
Correlative light and electron microscopy (CLEM) advances biological discoveries by merging different microscopes and imaging modalities to study systems in 4D. Combining fluorescence microscopy with…Read article
Crystal clear cryo light-microscopy images
This article describes how computational clearing of cryo light microscopy images improves the identification of cellular targets for cryo electron-microscopy.Read article
Microscopy in Virology
The coronavirus SARS-CoV-2, causing the Covid-19 disease effects our world in all aspects. Research to find immunization and treatment methods, in other words to fight this virus, gained highest…Read article
Introduction to Ion Beam Etching with the EM TIC 3X
In this article you can learn how to optimize the preparation quality of your samples by using the ion beam etching method with the EM TIC 3X ion beam milling machine. A short introduction of the…Read article
Improve Cryo Electron Tomography Workflow
Leica Microsystems and Thermo Fisher Scientific have collaborated to create a fully integrated cryo-tomography workflow that responds to these research needs: Reveal cellular mechanisms at…Read article
Correlative Cryo-Fluorescence and Cryo-Scanning Electron Microscopy as a Straightforward Tool to Study Host-Pathogen Interactions
Correlative light and electron microscopy is an imaging technique that enables identification and targeting of fluorescently tagged structures with subsequent imaging at near-to-nanometer resolution.…Read article
Imaging of Host Cell-bacteria Interactions using Correlative Microscopy under Cryo-conditions
Pathogenic bacteria have developed intriguing strategies to establish and promote infections in their respective hosts. Most bacterial pathogens initiate infectious diseases by adhering to host cells…Read article
Correlative In-Resin Super-Resolution and Electron Microscopy Using Standard Fluorescent Proteins
We introduce a method for correlative in-resin super-resolution fluorescence and electron microscopy (EM) of biological structures in mammalian culture cells. Cryo-fixed resin embedded samples offer…Read article
Correlative Light and Electron Microscopy – Get Your Free CLEM e-Book for Download
The urge to go deeper into the microscopic world has led researchers to combine the versatility of the light microscopy (LM) with the resolution power of the electron microscope (EM) to produce…Read article
Correlating Intravital Multi-Photon Microscopy to 3D Electron Microscopy of Invading Tumor Cells Using Anatomical Reference Points
Cancer research unsing multiphoton microscopy and 3D electron microscopy. Correlative microscopy combines the advantages of both light and electron microscopy to enable imaging of rare and transient…Read article
Customized Patterned Substrates for Highly Versatile Correlative Light-Scanning Electron Microscopy
Correlative light electron microscopy (CLEM) combines the advantages of light and electron microscopy, thus making it possible to follow dynamic events in living cells at nanometre resolution. Various…Read article
Useful Links
Communities
www.researchgate.net/Social network for scientists
bitesizebio.comOnline magazine and community for molecular and cell biology researchers
Search Engines and Data Bases
www.cellimagelibrary.orgPublic resource database of images, videos, and animations of cells
harvester.fzk.de/harvesterBioinformatic meta search engine for genes and proteins
www.gopubmed.comSearch interface for pubmed
en.wikipedia.org/wiki/List_of_academic_databases_and_search_enginesList of academic databases and search engines
scholar.google.comBeta of Google's search engine for scientific article abstracts
www.somersault1824.comResource for high-end scientific illustrations, images and animations
Web Sources
https://www.elsevier.com/books/correlative-light-and-electron-microscopy/unknown/978-0-12-416026-2Methods in Cell Biology – Vol. 111: Correlative Light and Electron Microscopy (Mueller-Reichert T, Verkade P)
http://my.safaribooksonline.com/book/-/9781118271926/chapter-6-toward-a-3d-view-of-cellular-architecture-correlative-light-microscopy/c06_level1_5_xhtml Biomedical Imaging: Principles and Applications (Salzer R), Chapter 6.5: CLEM Applications
https://openaccess.leidenuniv.nl/handle/1887/17922 Leiden University: Correlative Light and Electron Microscopy: Strategies and Applications
http://teemcof.ibp.cnr.it/services/correlative-light-electron-microscopyThe TeleThon Electron Microscopy Core Facility (TeEMCoF): Correlative Light-Electron Microscopy
www.embl.de/research/units/cbb/schwab/Volume Correlative Light and Electron Microscopy
www.emez.ethz.ch/education/pastevents/pastev/Correl_LMEM_rw.pdf Correlative Microscopy in Focus: Zoom-in Beyond LM …
http://jcb.rupress.org/content/192/1/111.full Correlated Fluorescence and 3D Electron Microscopy with High Sensitivity and Spatial Precision
www.cellbiology-utrecht.nl/second-menu-cmc/techniques.html Cell Microscopy Center: Techniques
www.biocenter.helsinki.fi/bi/em/emu_methods_clem.html Methods: Correlative Light Electron Microscopy
www.i-bioimaging.it/images/Alberto%20Luini.pdf Correlative Microscopy
http://jcs.biologists.org/content/early/2012/06/05/jcs.090027.full.pdf Advances in High-resolution Imaging – Techniques for Three-dimensional Imaging of Cellular Structures
www.sartre.ac.uk/sites/default/files/Verkade.pdf A Single Probe to Combine 2 Imaging Modalities
www.imaging-git.com/science/light-microscopy/clem-ultrathin-sections CLEM on Ultrathin Sections – From Micro- to Nanoscale
www.formatex.info/microscopy4/277-285.pdf Correlative Light and Electron Microscopic Exploration of Endocytic Organelles
www.emc2012.org.uk//documents/Abstracts/Abstracts/EMC2012_1163.pdf In and Out of Mitosis – Correlative Light and Electron Microscopy for Structural Cell Biology
http://users.unimi.it/~cima/bibliocorrelative/jmicroscopy2007.pdf Cryo-fluorescence Microscopy Facilitates Correlations between Light and Cryo-electron Microscopy and Reduces the Rate of Photobleaching
www.cgl.ucsf.edu/Research/auer/ Correlative Light and Electron Microscopy: Visualization of Molecular Machines in Their Native Cell and Tissue Context
www.labmate-online.com/articles/microscopy-and-microtechniques/4/paul_verkade/the_use_of_high-pressure_freezing_in_correlative_light_electron_microscopy/71/ The Use of High-pressure Freezing in Correlative Light Electron Microscopy
http://de.scribd.com/doc/156152708/Photooxidation-Technology-for-Correlative-Light-and-Electron-Microscopy Photooxidation Technology for Correlative Light and Electron Microscopy
http://walsworth.physics.harvard.edu/publications/2012_Glenn_ArXiv.pdf Multi-color Correlative Light and Electron Microscopy Using Nanoparticle Cathodoluminescence
Organizations
www.microscopy.org/Microscopy Society of America
www.eurmicsoc.org/European Microscopy Society
www.rms.org.uk/Royal Microscopical Society
www.ascb.org/ASCB American Society of Cell Biology
www.biologists.com/cob_activities.htmlthe company of biologists
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