Science Lab

Science Lab

The knowledge portal of Leica Microsystems offers scientific research and teaching material on the subjects of microscopy. The content is designed to support beginners, experienced practitioners and scientists alike in their everyday work and experiments. Explore interactive tutorials and application notes, discover the basics of microscopy as well as high-end technologies – become part of the Science Lab community and share your expertise!

Latest Articles

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Kaggle Competition for Multi-label Classification of Cell Organelles in Proteome Scale Human Protein Atlas Data
The Cell Atlas, a part of the Human Protein Atlas, was created by the group of Prof. Emma Lundberg at the SciLifeLab, KTH Royal Institute of…
Dec 09, 2018
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See More Than Just Your Image
Despite the emergence of new imaging methods in recent years, true 3D resolution is still achieved by Confocal Laser Scanning Microscopy (CLSM).…
Dec 04, 2018
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You Shall Not Pass! Time-gated Detection Takes Care of Endogenous Fluorescence in Plant Research.
Yutaka Kodama is Associate Professor at the Biosciences Education Research Center, Utsunomiya University, Japan. He graduated from Saga University,…
Nov 13, 2018
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Simultaneously Measuring Image Features and Resolution in Live-Cell STED Images
Reliable interpretation and quantification of cellular features in fluorescence microscopy requires an accurate estimate of microscope resolution.…
Nov 12, 2018
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MONENSIN SENSITIVITY1 (MON1)/CALCIUM CAFFEINE ZINC SENSITIVITY1 (CCZ1)
Programmed cell death (PCD)-triggered degradation of plant tapetum is essential for microspore development and pollen coat formation; however, little…
Oct 22, 2018
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Which Sensor is the Best for Confocal Imaging?
The Hybrid Photodetectors (HyD) are! Why that is the case is explained in this short Science Lab article.
Jul 16, 2018
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mTORC1 Promotes Proliferation of Immature Schwann Cells and Myelin Growth of Differentiated Schwann Cells
The myelination of axons is essential for neuronal wiring and normal nervous system functions. In the peripheral nervous system, Schwann cells (SCs)…
May 16, 2018
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High-Resolution 3D Imaging of Whole Organ after Clearing
Zebrafish testis has become a powerful model for reproductive biology of teleostean fishes and other vertebrates and encompasses multiple applications…
May 08, 2018
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FLIM FRET - Förster Resonance Energy Transfer
A typical application of FLIM is FLIM-FRET. FRET is a well-established technique to study molecular interactions. It scrutinizes protein binding and…
Mar 28, 2018
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FCS - Fluorescence Correlation Spectroscopy
FCS is a fluorescence-based measurement method. Fluorescent molecules passing through a strongly focused, fixed laser beam are excited for…
Mar 28, 2018
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FCCS - Fluorescence Cross-Correlation Spectroscopy
FCCS (Fluorescence Cross-Correlation Spectroscopy) can be measured using the Leica TCS SP8 FCS system. Similar to FCS , it analyzes fluorescence…
Mar 25, 2018
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SP FLIM - Spectral Fluorescence Lifetime Imaging
The SP8 FALCON is the ideal tool for spectral FLIM detection. No emission filters in front of the FLIM detectors are necessary. This grants a much…
Mar 25, 2018
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What is FLIM - Fluorescence Lifetime Imaging?
The fluorescence lifetime is a measure of how long a fluorophore remains on average in its excited state before returning to the ground state by…
Mar 25, 2018
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KChIP2 regulates the cardiac Ca²⁺ transient and myocyte contractility by targeting ryanodine receptor activity
Pathologic electrical remodeling and attenuated cardiac contractility are featured characteristics of heart failure. Coinciding with these remodeling…
Mar 07, 2018
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Abstracts of the 7th European Super-Resolution User-Club Meeting
The 7th Super-Resolution User Club Meeting was held in collaboration with Prof Pavel Hozák , at the Institute of Molecular Genetics of the ASCR in…
Dec 17, 2017
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Primary Beam Splitting Devices for Confocal Microscopes
Current fluorescence microscopy employs incident illumination which requires separation of illumination and emission light. The classical device…
May 16, 2017
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Pinhole Effect in Confocal Microscopes
When operating a confocal microscope, or when discussing features and parameters of such a device, we inescapably mention the pinhole and its…
Apr 26, 2017
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Human NK Cell Development Requires CD56-mediated Motility and Formation of the Developmental Synapse
While distinct stages of natural killer (NK) cell development have been defined, the molecular interactions that shape human NK cell maturation are…
Feb 15, 2017
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